{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3714, "factors": [{"GSM559507": {"cell line": "MCF-7/LTED", "compound": "ethanol"}}, {"GSM559507": {"cell line": "MCF-7/LTED", "compound": "ethanol"}}, {"GSM559507": {"cell line": "MCF-7/LTED", "compound": "ethanol"}}, {"GSM559510": {"cell line": "MCF-7/LTED", "compound": "fulvestrant"}}, {"GSM559510": {"cell line": "MCF-7/LTED", "compound": "fulvestrant"}}, {"GSM559510": {"cell line": "MCF-7/LTED", "compound": "fulvestrant"}}, {"GSM559513": {"cell line": "HCC-1428/LTED", "compound": "ethanol"}}, {"GSM559513": {"cell line": "HCC-1428/LTED", "compound": "ethanol"}}, {"GSM559513": {"cell line": "HCC-1428/LTED", "compound": "ethanol"}}, {"GSM559516": {"cell line": "HCC-1428/LTED", "compound": "fulvestrant"}}, {"GSM559516": {"cell line": "HCC-1428/LTED", "compound": "fulvestrant"}}, {"GSM559516": {"cell line": "HCC-1428/LTED", "compound": "fulvestrant"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. To model resistance to aromatase...", "pubmed_id": 22049316, "geo_gse_id": "E-GEOD-22533", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 12, "tags": ["breast", "estrogen", "hcc", "hormone"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-human-breast-6", "geo_id_plat": "E-GEOD-22533_A-AFFY-44", "name": "Transcription profiling by array of human breast cancer cell lines treated with fulvestrant", "created": "Sep.15, 2014", "summary": "A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. To model resistance to aromatase inhibitor (AI) therapy, long-term estrogen-deprived (LTED) derivatives of MCF-7 and HCC-1428 cells were generated through culture for 3 and 7 months under hormone-depleted conditions, respectively. These LTED cells showed sensitivity to the ER downregulator fulvestrant under hormone-depleted conditions, suggesting continued dependence upon ER signaling for hormone-independent growth. To evaluate the role of ER in hormone-independent growth, LTED cells were treated +/- 1 uM fulvestrant x 48 h before RNA was harvested for gene expression analysis. MCF-7/LTED and HCC-1428/LTED cells were treated with 10% DCC-FBS with or without the estrogen receptor antagonist drug fulvestrant for 48 hrs prior to RNA harvest for array analysis. Three replicates per condition.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22533", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22533/samples/"}