{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 6128, "factors": [{"GSM461424": {"CELL TYPE": "Multipotent progenitor"}}, {"GSM461424": {"CELL TYPE": "Multipotent progenitor"}}, {"GSM461428": {"CELL TYPE": "Common dendritic cell progenitor"}}, {"GSM461428": {"CELL TYPE": "Common dendritic cell progenitor"}}, {"GSM46143": {"CELL TYPE": "Conventional dendritic cell differentiated from amplified progenitor cells"}}, {"GSM46143": {"CELL TYPE": "Conventional dendritic cell differentiated from amplified progenitor cells"}}, {"GSM461452": {"CELL TYPE": "Plasmacytoid dendritic cell differentiated from amplified progenitor cells"}}, {"GSM461452": {"CELL TYPE": "Plasmacytoid dendritic cell differentiated from amplified progenitor cells"}}, {"GSM557486": {"CELL TYPE": "untreated common dendritic cell progenitor amplified from mouse bone marrow"}}, {"GSM557486": {"CELL TYPE": "untreated common dendritic cell progenitor amplified from mouse bone marrow"}}, {"GSM557488": {"CELL TYPE": "TGF-beta1 treated common dendritic cell progenitor amplified from mouse bone marrow for 4 hours"}}, {"GSM557488": {"CELL TYPE": "TGF-beta1 treated common dendritic cell progenitor amplified from mouse bone marrow for 4 hours"}}, {"GSM557490": {"CELL TYPE": "TGF-beta1 treated common dendritic cell progenitor amplified from mouse bone marrow for 8 hours"}}, {"GSM557490": {"CELL TYPE": "TGF-beta1 treated common dendritic cell progenitor amplified from mouse bone marrow for 8 hours"}}, {"GSM557492": {"CELL TYPE": "TGF-beta1 treated common dendritic cell progenitor amplified from mouse bone marrow for 12 hours"}}, {"GSM557492": {"CELL TYPE": "TGF-beta1 treated common dendritic cell progenitor amplified from mouse bone marrow for 12 hours"}}, {"GSM557494": {"CELL TYPE": "TGF-beta1 treated common dendritic cell progenitor amplified from mouse bone marrow for 24 hours"}}, {"GSM557494": {"CELL TYPE": "TGF-beta1 treated common dendritic cell progenitor amplified from mouse bone marrow for 24 hours"}}, {"GSM557496": {"CELL TYPE": "In vivo common dendritic cell progenitor isolated from mouse bone marrow"}}, {"GSM557496": {"CELL TYPE": "In vivo common dendritic cell progenitor isolated from mouse bone marrow"}}], "pop_total": 0, "platform": 6, "summary_wrapped": "Dendritic cells (DCs) in lymphoid tissue comprise conventional DCs (cDCs) and plasmacytoid DCs (pDCs) that develop from common DC...", "pubmed_id": 20881193, "geo_gse_id": "E-GEOD-22432", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 20, "tags": ["bone", "bone marrow", "cell", "dendritic", "genome", "insulin", "lymphoid tissue", "stem cell", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-22432_A-AFFY-45", "slug": "tgf-1-accelerates-dendritic-cell-differentiation-f", "geo_gds_id": "", "name": "TGF-\u03b21 Accelerates Dendritic Cell Differentiation from Common Dendritic Cell Progenitors (CDPs) and Directs Subset Specification Towards Conventional Dendritic Cells", "created": "Nov.11, 2014", "summary": "Dendritic cells (DCs) in lymphoid tissue comprise conventional DCs (cDCs) and plasmacytoid DCs (pDCs) that develop from common DC progenitors (CDPs). CDPs are Flt3+c-kitintM-CSFR+ and reside in bone marrow. Here we describe a two-step culture system that recapitulates DC development from c-kithiFlt3-/lo multipotent progenitors (MPPs) into CDPs and further into cDC and pDC subsets. MPPs and CDPs are amplified in vitro with Flt3 ligand, stem cell factor, hyper-IL-6 and insulin- like growth factor-1. The four-factor cocktail readily induces self-renewal of MPPs and their progression into CDPs and has no self-renewal activity on CDPs. The amplified CDPs respond to all known DC poietins and generate all lymphoid tissue DCs in vivo and in vitro. Additionally, in vitro CDPs recapitulate the cell surface marker and gene expression profile of in vivo CDPs and possess a DC-primed transcription profile. Transforming growth factor-\u03b21 (TGF-\u03b21) impacts on CDPs and directs their differentiation towards cDCs. Genome-wide gene expression profiling of TGF-\u03b21-induced genes identified transcription factors, such as interferon regulatory factor-4 (IRF-4) and RelB, that are implicated as instructive factors for cDC subset specification. TGF-\u03b21 also induced the transcription factor inhibitor of differentiation/DNA binding 2 (Id2) that suppresses pDC development. Thus, TGF-\u03b21 directs CDP differentiation into cDC by inducing both cDC instructive factors and pDC inhibitory factors. 20 samples in total.  Multipotent progenitor - MPP_1 - MPP_2 Common dendritic cell progenitor - CDP_1 - CDP_2 Plasmacytoid dendritic cell - pDC_1 - pDC_2 Conventional dendritic cell - cDC_1 - cDC_2 In vivo common dendritic cell progenitor  - In vivo CDP_1 - In vivo CDP_2  Untreated common dendritic cell progenitor (CDP)  - CDP_0h_1 - CDP_0h_2 TGF-beta1 treated (4 hours) CDP - CDP_4h_1 - CDP_4h_2 TGF-beta1 treated (8 hours) CDP - CDP_8h_1 - CDP_8h_2 TGF-beta1 treated (12 hours) CDP - CDP_12h_1 - CDP_12h_2 TGF-beta1 treated (24 hours) CDP - CDP_24h_1 - CDP_24h_2", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22432", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22432/samples/"}