{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3702, "factors": [{"GSM556646": {"STRESS": "unstimulated", "CELL TYPE": "Macrophage"}}, {"GSM556647": {"STRESS": "unstimulated", "CELL TYPE": "Monocyte"}}, {"GSM556646": {"STRESS": "unstimulated", "CELL TYPE": "Macrophage"}}, {"GSM556647": {"STRESS": "unstimulated", "CELL TYPE": "Monocyte"}}, {"GSM556664": {"STRESS": "stimulated with Flagellin", "CELL TYPE": "Macrophage"}}, {"GSM556665": {"STRESS": "stimulated with Flagellin", "CELL TYPE": "Monocyte"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Human intestinal macrophages contribute to tissue homeostasis in noninflamed mucosa through profound down-regulation of pro-inflammatory...", "pubmed_id": 20388715, "geo_gse_id": "E-GEOD-22373", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 6, "tags": ["cytokine", "interleukin", "mucosa", "stroma"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "monocyte-vs-macrophage-study", "geo_id_plat": "E-GEOD-22373_A-AFFY-44", "name": "Monocyte vs Macrophage Study", "created": "Sep.15, 2014", "summary": "Human intestinal macrophages contribute to tissue homeostasis in noninflamed mucosa through profound down-regulation of pro-inflammatory cytokine release. Here, we show that this down-regulation extends to Toll-like receptor (TLR)-induced cytokine release, as intestinal macrophages expressed TLR3-TLR9 but did not release cytokines in response to TLR-specific ligands. Likely contributing to this unique functional profile, intestinal macrophages expressed markedly down-regulated adapter proteins MyD88 and Toll interleukin receptor 1 domain-containing adapter-inducing interferon beta, which together mediate all TLR MyD88-dependent and -independent NF-kappaB signaling, did not phosphorylate NF-kappaB p65 or Smad-induced IkappaBalpha, and did not translocate NF-kappaB into the nucleus. Importantly, transforming growth factor-beta released from intestinal extracellular matrix (stroma) induced identical down-regulation in the NF-kappaB signaling and function of blood monocytes, the exclusive source of intestinal macrophages. Our findings implicate stromal transforming growth factor-beta-induced dysregulation of NF-kappaB proteins and Smad signaling in the differentiation of pro-inflammatory blood monocytes into noninflammatory intestinal macrophages. Comparison of unstimulated monocytes and macrophages, and flagellin stimulated monocytes and macrophages.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22373", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22373/samples/"}