ArrayExpress Uploader0humansimulated 4h with % 0.01 BSAsimulated 8h with % 0.01 BSAsimulated 16h with % 0.01 BSAsimulated 48h with % 0.01 BSAsimulated 4h with IL-1beta(100 U)simulated 8h with IL-1beta(100 U)simulated 16h with IL-1beta(100 U)simulated 4h with IL-3(100 U)simulated 8h with IL-3(100 U)simulated 16h with IL-3(100 U)simulated 48h with IL-3(100 U)simulated 4h with IL-6(100 U)simulated 8h with IL-6(100 U)simulated 16h with IL-6(100 U)simulated 48h with IL-6(100 U)non-stimulated, 0h biological rep 1non-stimulated, 0h biological rep 2non-stimulated, 16h3695arrayexpress_sid4In the hematopoietic microenvironment, endothelial cells (ECs) play an important role in the regulation of hematopoietic cell...E-GEOD-22325/profile/8773/arrayexpressuploader118celldendritichematopoietic cellpointumbilical veinveinDec.12, 2014Falseexpression-data-from-interleukin-stimulated-huvecE-GEOD-22325_A-AFFY-44Expression data from interleukin-stimulated HUVECSep.15, 2014In the hematopoietic microenvironment, endothelial cells (ECs) play an important role in the regulation of hematopoietic cell proliferation and trafficking. We previously demonstrated that EC stimulated with tumor necrosis factor alpha (TNF-α) induce the generation of dendritic cells from CD34(+) stem cells, whereas in contrast, interleukins were capable of inducing the proliferation of hematopoietic and myeloid progenitors. In order to identify potentially new soluble factors which greatly impact the self-renewal, proliferation and differentiation of CD34+ hematopoietic stem cells (HSC), we examined the expression profiles of IL-1ß, IL-3 and IL-6 stimulated human umbilical vein endothelial cells (HUVEC). we processed seven different umbilical cords and isolated 129 samples for total RNA, and pooled them into 18 groups corresponding to each stimulant, control and time point.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22325http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22325/samples/