BioGPS
  • Home
  • Help
  • Plugins
  • Datasets
  • Sign Up
  • Login
Examples: Gene Symbol(s), Gene Ontology, Splicing plugins, Melanoma datasets
advanced
Home › Dataset Library › Timecourse expression data from sciatic nerve distal stump of C57BL/6 and C57BL/6 OlaHsd-Wlds mice.

Dataset: Timecourse expression data from sciatic nerve distal stump of C57BL/6 and C57BL/6 OlaHsd-Wlds mice.

Wallerian degeneration (WD) involves the fragmentation of axonal segments disconnected from their cell bodies, segmentation of the myelin...

Registered by ArrayExpress Uploader
View Dataset

Wallerian degeneration (WD) involves the fragmentation of axonal segments disconnected from their cell bodies, segmentation of the myelin sheath, and removal of debris by Schwann cells and immune cells. The removal and downregulation of myelin-associated inhibitors of axonal regeneration and synthesis of growth factors by these two cell types are critical responses to successful nerve repair. Here, we analyzed the transcriptome of the sciatic nerve of mice carrying the Wallerian degeneration slow (WldS) mutant gene, a gene that confers axonal protection in the distal stump after injury, therefore causing significant delays in WD, neuroinflammation, and axonal regeneration. 56 C57BL6 mice and 56 C57BL/6 OlaHsd-Wlds mice were anesthetized with isoflurane and underwent a microcrush lesion of their left sciatic nerve at the mid-thigh level (exept naive mice, t0). At 0, 3, 7 and 14 days post-injury, mice were anesthetized and killed by cervical dislocation. Sciatic nerves were collected and conective tissue removed. A 4-mm long sciatic nerve segment was taken from the nerve distal stump, starting at 1 mm distal from the lesion up to 5 mm distal. Distal nerve stumps were pooled by group and RNA extracted. Samples were hybridized to GeneChip® Mouse Genome 430 2.0 Array (Affymetrix). Biological replicate was done.

Species:
mouse

Samples:
16

Source:
E-GEOD-22291

PubMed:
20688153

Updated:
Dec.12, 2014

Registered:
Nov.11, 2014


Factors: (via ArrayExpress)
Sample STRAIN STRESS GENOTYPE/VARIATION TIME
GSM554762 C57BL/6 uninjured (naive) wild type 0 days
GSM554762 C57BL/6 uninjured (naive) wild type 0 days
GSM554764 C57BL/6 injured (microcrush lesion) wild type 3 days (post-injury)
GSM554764 C57BL/6 injured (microcrush lesion) wild type 3 days (post-injury)
GSM554766 C57BL/6 injured (microcrush lesion) wild type 7 days (post-injury)
GSM554766 C57BL/6 injured (microcrush lesion) wild type 7 days (post-injury)
GSM554768 C57BL/6 injured (microcrush lesion) wild type 14 days (post-injury)
GSM554768 C57BL/6 injured (microcrush lesion) wild type 14 days (post-injury)
GSM554770 C57BL/6 OlaHsd-Wlds uninjured (naive) WldS mutant 0 days
GSM554770 C57BL/6 OlaHsd-Wlds uninjured (naive) WldS mutant 0 days
GSM554772 C57BL/6 OlaHsd-Wlds injured (microcrush lesion) WldS mutant 3 days (post-injury)
GSM554772 C57BL/6 OlaHsd-Wlds injured (microcrush lesion) WldS mutant 3 days (post-injury)
GSM554774 C57BL/6 OlaHsd-Wlds injured (microcrush lesion) WldS mutant 7 days (post-injury)
GSM554774 C57BL/6 OlaHsd-Wlds injured (microcrush lesion) WldS mutant 7 days (post-injury)
GSM554776 C57BL/6 OlaHsd-Wlds injured (microcrush lesion) WldS mutant 14 days (post-injury)
GSM554776 C57BL/6 OlaHsd-Wlds injured (microcrush lesion) WldS mutant 14 days (post-injury)

Tags

  • cell
  • distal
  • genome
  • left
  • left sciatic nerve
  • myelin
  • myelin sheath
  • nerve
  • sciatic nerve
  • segmentation
  • thigh

Other Formats

JSON    XML
  • About
  • Blog
  • Help
  • FAQ
  • Downloads
  • API
  • iPhone App
  • Email updates
© 2019 The Scripps Research Institute. All rights reserved. (ver 59fce91 )
  • Terms of Use