ArrayExpress Uploader0human20.674.282.1670.2058.2330.4144.163.848.0430.373.643.718.0893.341.462.0344.9592.40kidney transplant recipient1.929.910.9829.162.8481.712.78SRL monotherapy0.180.6229.930.773.442.2515.049.541.0125.8748.7439.2153.665.5028.7345.446.728.7115.1180.541.944.8038.8888.11kidney transplant recipient1.927.230.231.5215.9055.1510.39SRL monotherapy1.460.4663.823.420.329.323.4520.714.8538.2954.0429.9951.9942.7917.2640.466.706.1434.5253.962.472.0510.6896.76kidney transplant recipient4.1537.230.1015.258.6243.3714.59SRL monotherapy1.920.816.132.560.4236.7822.206.0710.6513.0751.5269.6335.3118.7517.0644.5859.284.1613.4233.243.0947.2740.2894.14kidney transplant recipient1.6916.388.035.422.2258.865.60SRL monotherapy1.271.5320.891.771.25.7134.838.4014.1331.7168.2146.2432.0231.9812.816.6920.854.2725.8056.950.8110.9737.2596.22kidney transplant recipient3.0824.546.1917.275.3747.263.53SRL monotherapy2.220.4125.332.630.1819.575.0413.790.6828.0156.362.3849.959.1030.4337.606.516.349.9084.166.784.4841.9792.93kidney transplant recipient2.288.040.0411.646.9258.999.90SRL monotherapy6.70.2758.413.370.045.974.274.271.2319.2771.813.5843.5044.0426.6422.937.89 31.4448.623.254.3825.3995.04kidney transplant recipient2.9430.890.224.443.5840.694.40SRL monotherapy2.191.0730.748.990.4934.4929.418.696.248.2250.7237.9434.7315.3417.2344.5167.646.4126.8827.185.1440.3335.5194.30kidney transplant recipient1.6326.463.308.871.2249.653.50SRL monotherapy0.484.4836.100.659.334.5321.148.724.845.7833.607.0118.836.3634.1452.2648.901.1159.7042.003.638.1120.6195.66kidney transplant recipient0.5811.7648.8087.181.351.322.69CsA monotherapy1.033.0849.752.802.996.3014.664.592.269.7366.0111.1344.5020.9627.1325.0271.226.8337.1120.36.3656.7815.8795.36kidney transplant recipient3.1339.090.546.6217.9033.494.73CsA monotherapy0.454.7513.5332.610.55555555555565.845.0916.812.2327.9461.7155.4655.0712.7831.6431.9914.772.1916.5966.224.2712.9928.6495.43kidney transplant recipient1.6415.680.619.283.5549.543.20SRL monotherapy4.110.2946.307.030.0711.987.926.630.0721.4759.5234.9542.0233.7981.4334.1039.144.0014.8750.902.915.6247.3891.42kidney transplant recipient11.7210.270.338.945.013.635.72SRL monotherapy2.10.329.123.500.146.4410.586.221.179.5742.2663.2447.4311.6017.7846.8228.374.242.2465.027.7127.9249.4186.28kidney transplant recipient7.702.920.2418.273.9978.817.38SRL monotherapy1.51.5750.914.551.052.0610.9012.526.9210.6462.3812.7137.8432.1020.5631.8538.044.4545.5733.844.7329.0114.0695.13kidney transplant recipient3.0647.290.8111.429.6526.953.37SRL monotherapy1.634.9328.252.093.0226.0326.6612.578.284.6239.0348.0744.205.7626.6458.2259.0510.8011.3937.873.6952.9936.4694.08kidney transplant recipient1.2114.674.678.074.6455.691.23CsA monotherapy0.791.9336.630.772.442.3134.158.451.317.6739.275.6735.262.6125.6753.2125.133.4811.5773.624.6626.4552.6394.51kidney transplant recipient1.8711.600.5112.482.4361.462.06SRL monotherapy1.413.0663.270.632.170.6246.027.9039.505.4832.892.3926.0474.0715.2761.2690.132.3022.716.883.1617.9910.4095.43kidney transplant recipient1.3458.365.205.062.4222.523.97SRL monotherapy1.861.182.450.300.632.6928.9216.161.4129.0736.8048.6765.9520.2717.1656.3716.262.829.4882.297.519.7616.4292.41kidney transplant recipient1.7617.540.0910.160.6571.966.96CsA monotherapy1.984.5140.910.362.281.0912.7812.731.8416.5355.600.9436.7519.3428.9039.3144.864.1546.2439.796.7535.9916.3476.41kidney transplant recipient 45.821.094.066.0223.023.50CsA monotherapy1.684.9328.143.902.9311.45 kidney transplant recipient SRL monotherapy 18.146.631.2110.0762.5156.9618.2615.3511.4330.9824.977.8120.4862.673.4512.7059.7395.78kidney transplant recipient0.8321.680.3338.183.0966.882.03CsA monotherapy2.411.1961.381.440.4910.9111.4412.250.638.7261.3610.8453.0723.5542.2633.7125.782.7412.4452.384.6922.3634.8882.41kidney transplant recipient 12.040.0110.246.1144.674.95SRL monotherapy3.60.7645.373.560.2118.2812.078.8721.819.9961.843.1539.9014.4912.8731.6553.634.901.1743.764.141.5638.5294.76kidney transplant recipient4.8311.909.6833.016.8064.153.86CsA monotherapy2.061.8933.960.940.921.6610.447.712.9710.9931.7737.0138.1449.2329.0559.4137.781.6548.9728.757.8216.4918.4685.06kidney transplant recipient3.5142.960.4413.178.8619.011.65CsA monotherapy3.384.9123.304.221.4529.252.179.421.3321.9280.2360.7151.4648.1238.6415.8616.184.0045.0731.743.938.567.5695.30kidney transplant recipient1.0940.850.131.553.8414.963.01SRL monotherapy3.630.1921.4010.070.0542.0122.2417.430.8310.3640.085.8331.986.0315.5455.6935.5915.313.0360.591.9736.7064.7693.73kidney transplant recipient2.242.840.425.719.3280.604.94SRL monotherapy1.330.3346.920.680.253.151.427.550.3729.8483.898.9758.9130.5745.8314.5921.815.6729.0348.091.085.7918.2796.73kidney transplant recipient1.2722.800.021.9715.1724.786.66SRL monotherapy0.790.1433.8010.640.1819.464.9210.860.7122.4168.6932.0045.4142.7527.4924.8012.785.3232.6750.114.496.3621.6094.26kidney transplant recipient1.4232.900.092.5111.0539.138.38SRL monotherapy2.920.9928.484.820.3432.284.7014.882.3026.3964.0119.8449.6021.6322.4631.1315.634.1021.5164.572.6310.2131.1693.64kidney transplant recipient5.7518.590.658.3312.4953.722.00CsA monotherapy1.610.7941.784.720.4915.099.5219.411.0214.3945.6640.4150.8243.9434.9647.9814.552.0930.5342.865.1712.6120.6793.67kidney transplant recipient4.7228.470.047.027.3233.504.23SRL monotherapy3.791.529.074.200.438.397.846.890.5414.9074.5762.6950.6827.4740.7623.3235.378.9735.3143.790.915.1620.2797.44kidney transplant recipient1.5028.970.083.562.8523.389.81CsA monotherapy1.550.4442.472.940.2817.908.418.891.2625.1770.6929.0956.0715.8937.6126.5722.441.8918.4057.762.3816.5026.0693.88kidney transplant recipient2.9117.580.293.9211.1142.734.42CsA monotherapy0.191.5142.457.237.9512.578.959.641.6212.4260.272.9441.8211.2332.8727.0024.391.3334.9461.1311.7321.9322.5086.74kidney transplant recipient2.6735.110.5712.648.7930.576.04CsA monotherapy10.521.3954.436.590.137.9011.826.531.2720.8560.0737.4950.1919.2430.5335.0627.692.2630.2952.507.729.8417.6180.19kidney transplant recipient2.8531.880.323.5511.3937.906.25CsA monotherapy1.445.9230.077.114.1112.7017.8010.4729.960.7654.8339.2224.4829.8511.2537.2484.266.9134.672.995.6467.6917.1089.07kidney transplant recipient1.4540.5817.846.821.0124.128.14CsA monotherapy3.891.811.690.320.4712.434.9818.360.6730.2253.9041.6550.1334.0335.7031.616.341.8832.5156.916.963.8117.4778.38kidney transplant recipient 32.260.145.016.2031.126.14SRL monotherapy6.30.9231.944.830.1531.922.5717.522.328.3865.6510.6644.6274.1729.6830.318.314.9744.3224.972.84.2110.7694.37kidney transplant recipient2.5944.420.205.219.7023.699.65SRL monotherapy1.821.2813.233.820.762.89 Healthy volunteer none Healthy volunteer none Healthy volunteer none Healthy volunteer none Healthy volunteer none Healthy volunteer none Healthy volunteer none Healthy volunteer none Healthy volunteer none 3686arrayexpress_sid4Due to its low level of nephrotoxicity and capacity to harness tolerogenic pathways, sirolimus (SRL) has been proposed as an alternative...E-GEOD-22224/profile/8773/arrayexpressuploader3346cellimmune systemkidneymonocyteDec.12, 2014Falsecomparative-transcriptional-and-phenotypic-peripheE-GEOD-22224_A-AFFY-44Comparative Transcriptional and Phenotypic Peripheral Blood Analysis of Kidney Recipients under Cyclosporin A or Sirolimus MonotherapySep.15, 2014Due to its low level of nephrotoxicity and capacity to harness tolerogenic pathways, sirolimus (SRL) has been proposed as an alternative to calcineurin inhibitors in transplantation. The exact mechanisms underlying its unique immunosuppressive profile in humans, however, are still not well understood. In the current study we aimed to depict the in vivo effects of SRL in comparison with cyclosporin A (CSA) by employing gene expression profiling and multiparameter flow cytometry on blood cells collected from stable kidney recipients under immunosuppressant monotherapy. SRL recipients displayed an increased frequency of CD4+CD25highFoxp3+ T cells. However, this was accompanied by an increased number of effector memory T cells and by enrichment in NFkB-related pro-inflammatory expression pathways and monocyte and NK cell lineage-specific transcripts. Furthermore, measurement of a transcriptional signature characteristic of operationally tolerant kidney recipients failed to detect differences between SRL and CSA treated recipients. In conclusion, we show here that the blood transcriptional profile induced by SRL monotherapy in vivo does not resemble that of operationally tolerant recipients and is dominated by innate immune cells and NFkB-related pro-inflammatory events. These data provide novel insights on the complex effects of SLR on the immune system in clinical transplantation. The complete database comprised the expression measurements of 54 675 genes for kidney trasplant recipient PBMC treated with SRL (n=23) and CSA (n=14) monotherapy. 9 healthy controls were also included.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22224http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22224/samples/