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Home › Dataset Library › Comparative Transcriptional and Phenotypic Peripheral Blood Analysis of Kidney Recipients under Cyclosporin A or Sirolimus Monotherapy

Dataset: Comparative Transcriptional and Phenotypic Peripheral Blood Analysis of Kidney Recipients under Cyclosporin A or Sirolimus Monotherapy

Due to its low level of nephrotoxicity and capacity to harness tolerogenic pathways, sirolimus (SRL) has been proposed as an alternative...

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Due to its low level of nephrotoxicity and capacity to harness tolerogenic pathways, sirolimus (SRL) has been proposed as an alternative to calcineurin inhibitors in transplantation. The exact mechanisms underlying its unique immunosuppressive profile in humans, however, are still not well understood. In the current study we aimed to depict the in vivo effects of SRL in comparison with cyclosporin A (CSA) by employing gene expression profiling and multiparameter flow cytometry on blood cells collected from stable kidney recipients under immunosuppressant monotherapy. SRL recipients displayed an increased frequency of CD4+CD25highFoxp3+ T cells. However, this was accompanied by an increased number of effector memory T cells and by enrichment in NFkB-related pro-inflammatory expression pathways and monocyte and NK cell lineage-specific transcripts. Furthermore, measurement of a transcriptional signature characteristic of operationally tolerant kidney recipients failed to detect differences between SRL and CSA treated recipients. In conclusion, we show here that the blood transcriptional profile induced by SRL monotherapy in vivo does not resemble that of operationally tolerant recipients and is dominated by innate immune cells and NFkB-related pro-inflammatory events. These data provide novel insights on the complex effects of SLR on the immune system in clinical transplantation. The complete database comprised the expression measurements of 54 675 genes for kidney trasplant recipient PBMC treated with SRL (n=23) and CSA (n=14) monotherapy. 9 healthy controls were also included.

Species:
human

Samples:
46

Source:
E-GEOD-22224

Updated:
Dec.12, 2014

Registered:
Sep.15, 2014


Factors: (via ArrayExpress)
Sample %CD8+CD28- %CD8+CD28+ %CD4 CCR7 - CD45+ %CD8 CD62L+ CD45- %CD4 CCR7+ CD45+ %CD4CD25CD62L %CD3-CD56+CD16+ %CD4CD62L+CD45- %CD8CD62L+ CD45+ %CD4 CCR7+ CD45 - %CD3CD8 %CD8CCR7-CD45+ %CD8CD62L- CD45 - %CD3CD4 %CD8CCR7-CD45 - %CD3DELTA1 %CD8CD62L- CD45+ %CD4 CD62L - CD45- %CD3ALPHABETA %NKTS %CD4CD8-AB+(DN) %CD4 CD62L-CD45+ %CD4 CD62L+CD45+ %CD19 %CD4 CCR7 – CD45- %CD4FOXP3+ THERAPY %CD3DELTA2 %CD3GAMMADELTA %CD8CCR7+ CD45- RATIO DELTA1/DELTA2 DISEASE STATUS %CD8CCR7+ CD45+
GSM553442 20.67 4.28 2.16 70.20 8.08 3.71 30.41 44.16 3.84 8.04 30.37 3.64 29.93 58.23 93.34 0.62 2.03 44.95 92.40 29.16 1.92 0.98 9.91 2.84 81.71 2.78 SRL monotherapy 0.18 1.46 0.77 3.44 kidney transplant recipient 2.25
GSM553443 15.04 9.54 1.01 25.87 15.11 8.71 39.21 53.66 5.50 28.73 45.44 6.72 63.82 48.74 80.54 0.46 4.80 38.88 88.11 1.52 1.92 0.23 7.23 15.90 55.15 10.39 SRL monotherapy 1.46 1.94 3.42 0.32 kidney transplant recipient 9.32
GSM553444 3.45 20.71 4.85 38.29 34.52 6.14 29.99 51.99 42.79 17.26 40.46 6.70 16.13 54.04 53.96 0.8 2.05 10.68 96.76 15.25 4.15 0.10 37.23 8.62 43.37 14.59 SRL monotherapy 1.92 2.47 2.56 0.42 kidney transplant recipient 36.78
GSM553445 22.20 6.07 10.65 13.07 13.42 4.16 69.63 35.31 18.75 17.06 44.58 59.28 20.89 51.52 33.24 1.53 47.27 40.28 94.14 5.42 1.69 8.03 16.38 2.22 58.86 5.60 SRL monotherapy 1.27 3.09 1.77 1.2 kidney transplant recipient 5.71
GSM553446 34.83 8.40 14.13 31.71 25.80 4.27 46.24 32.02 31.98 12.81 6.69 20.85 25.33 68.21 56.95 0.41 10.97 37.25 96.22 17.27 3.08 6.19 24.54 5.37 47.26 3.53 SRL monotherapy 2.22 0.81 2.63 0.18 kidney transplant recipient 19.57
GSM553447 5.04 13.79 0.68 28.01 9.90 6.34 2.38 49.95 9.10 30.43 37.60 6.51 58.41 56.36 84.16 0.27 4.48 41.97 92.93 11.64 2.28 0.04 8.04 6.92 58.99 9.90 SRL monotherapy 6.7 6.78 3.37 0.04 kidney transplant recipient 5.97
GSM553448 4.27 4.27 1.23 19.27 31.44 3.58 43.50 44.04 26.64 22.93 7.89 30.74 71.81 48.62 1.07 4.38 25.39 95.04 4.44 2.94 0.22 30.89 3.58 40.69 4.40 SRL monotherapy 2.19 3.25 8.99 0.49 kidney transplant recipient 34.49
GSM553449 29.41 8.69 6.24 8.22 26.88 6.41 37.94 34.73 15.34 17.23 44.51 67.64 36.10 50.72 27.18 4.48 40.33 35.51 94.30 8.87 1.63 3.30 26.46 1.22 49.65 3.50 SRL monotherapy 0.48 5.14 0.65 9.33 kidney transplant recipient 4.53
GSM553450 21.14 8.72 4.84 5.78 59.70 1.11 7.01 18.83 6.36 34.14 52.26 48.90 49.75 33.60 42.00 3.08 38.11 20.61 95.66 87.18 0.58 48.80 11.76 1.35 1.32 2.69 CsA monotherapy 1.03 3.6 2.80 2.99 kidney transplant recipient 6.30
GSM55345 14.66 4.59 2.26 9.73 37.11 6.83 11.13 44.50 20.96 27.13 25.02 71.22 13.533 66.01 20.3 4.75 56.78 15.87 95.36 6.62 3.13 0.54 39.09 17.90 33.49 4.73 CsA monotherapy 0.45 6.36 2.6 10.5555555555556 kidney transplant recipient 5.84
GSM553452 5.09 16.81 2.23 27.94 16.59 2.19 55.46 55.07 12.78 31.64 31.99 14.77 46.30 61.71 66.22 0.29 12.99 28.64 95.43 9.28 1.64 0.61 15.68 3.55 49.54 3.20 SRL monotherapy 4.11 4.27 7.03 0.07 kidney transplant recipient 11.98
GSM553453 7.92 6.63 0.07 21.47 14.87 4.00 34.95 42.02 33.79 81.43 34.10 39.14 29.12 59.52 50.90 0.3 15.62 47.38 91.42 8.94 11.72 0.33 10.27 5.01 3.63 5.72 SRL monotherapy 2.1 2.9 3.50 0.14 kidney transplant recipient 6.44
GSM553454 10.58 6.22 1.17 9.57 2.24 4.24 63.24 47.43 11.60 17.78 46.82 28.37 50.91 42.26 65.02 1.57 27.92 49.41 86.28 18.27 7.70 0.24 2.92 3.99 78.81 7.38 SRL monotherapy 1.5 7.71 4.55 1.05 kidney transplant recipient 2.06
GSM553455 10.90 12.52 6.92 10.64 45.57 4.45 12.71 37.84 32.10 20.56 31.85 38.04 28.25 62.38 33.84 4.93 29.01 14.06 95.13 11.42 3.06 0.81 47.29 9.65 26.95 3.37 SRL monotherapy 1.63 4.73 2.09 3.02 kidney transplant recipient 26.03
GSM553456 26.66 12.57 8.28 4.62 11.39 10.80 48.07 44.20 5.76 26.64 58.22 59.05 36.63 39.03 37.87 1.93 52.99 36.46 94.08 8.07 1.21 4.67 14.67 4.64 55.69 1.23 CsA monotherapy 0.79 3.69 0.77 2.44 kidney transplant recipient 2.31
GSM553457 34.15 8.45 1.31 7.67 11.57 3.48 5.67 35.26 2.61 25.67 53.21 25.13 63.27 39.27 73.62 3.06 26.45 52.63 94.51 12.48 1.87 0.51 11.60 2.43 61.46 2.06 SRL monotherapy 1.41 4.66 0.63 2.17 kidney transplant recipient 0.62
GSM553458 46.02 7.90 39.50 5.48 22.71 2.30 2.39 26.04 74.07 15.27 61.26 90.13 2.45 32.89 6.88 1.18 17.99 10.40 95.43 5.06 1.34 5.20 58.36 2.42 22.52 3.97 SRL monotherapy 1.86 3.16 0.30 0.63 kidney transplant recipient 2.69
GSM553459 28.92 16.16 1.41 29.07 9.48 2.82 48.67 65.95 20.27 17.16 56.37 16.26 40.91 36.80 82.29 4.51 9.76 16.42 92.41 10.16 1.76 0.09 17.54 0.65 71.96 6.96 CsA monotherapy 1.98 7.51 0.36 2.28 kidney transplant recipient 1.09
GSM553460 12.78 12.73 1.84 16.53 46.24 4.15 0.94 36.75 19.34 28.90 39.31 44.86 28.14 55.60 39.79 4.93 35.99 16.34 76.41 4.06 1.09 45.82 6.02 23.02 3.50 CsA monotherapy 1.68 6.75 3.90 2.93 kidney transplant recipient 11.45
GSM55346 SRL monotherapy kidney transplant recipient
GSM553462 18.14 6.63 1.21 10.07 20.48 7.81 56.96 18.26 15.35 11.43 30.98 24.97 61.38 62.51 62.67 1.19 12.70 59.73 95.78 38.18 0.83 0.33 21.68 3.09 66.88 2.03 CsA monotherapy 2.41 3.45 1.44 0.49 kidney transplant recipient 10.91
GSM553463 11.44 12.25 0.63 8.72 12.44 2.74 10.84 53.07 23.55 42.26 33.71 25.78 45.37 61.36 52.38 0.76 22.36 34.88 82.41 10.24 0.01 12.04 6.11 44.67 4.95 SRL monotherapy 3.6 4.69 3.56 0.21 kidney transplant recipient 18.28
GSM553464 12.07 8.87 21.81 9.99 1.17 4.90 3.15 39.90 14.49 12.87 31.65 53.63 33.96 61.84 43.76 1.89 41.56 38.52 94.76 33.01 4.83 9.68 11.90 6.80 64.15 3.86 CsA monotherapy 2.06 4.1 0.94 0.92 kidney transplant recipient 1.66
GSM553465 10.44 7.71 2.97 10.99 48.97 1.65 37.01 38.14 49.23 29.05 59.41 37.78 23.30 31.77 28.75 4.91 16.49 18.46 85.06 13.17 3.51 0.44 42.96 8.86 19.01 1.65 CsA monotherapy 3.38 7.82 4.22 1.45 kidney transplant recipient 29.25
GSM553466 2.17 9.42 1.33 21.92 45.07 4.00 60.71 51.46 48.12 38.64 15.86 16.18 21.40 80.23 31.74 0.19 8.56 7.56 95.30 1.55 1.09 0.13 40.85 3.84 14.96 3.01 SRL monotherapy 3.63 3.93 10.07 0.05 kidney transplant recipient 42.01
GSM553467 22.24 17.43 0.83 10.36 3.03 15.31 5.83 31.98 6.03 15.54 55.69 35.59 46.92 40.08 60.59 0.33 36.70 64.76 93.73 5.71 2.24 0.42 2.84 9.32 80.60 4.94 SRL monotherapy 1.33 1.97 0.68 0.25 kidney transplant recipient 3.15
GSM553468 1.42 7.55 0.37 29.84 29.03 5.67 8.97 58.91 30.57 45.83 14.59 21.81 33.80 83.89 48.09 0.14 5.79 18.27 96.73 1.97 1.27 0.02 22.80 15.17 24.78 6.66 SRL monotherapy 0.79 1.08 10.64 0.18 kidney transplant recipient 19.46
GSM553469 4.92 10.86 0.71 22.41 32.67 5.32 32.00 45.41 42.75 27.49 24.80 12.78 28.48 68.69 50.11 0.99 6.36 21.60 94.26 2.51 1.42 0.09 32.90 11.05 39.13 8.38 SRL monotherapy 2.92 4.49 4.82 0.34 kidney transplant recipient 32.28
GSM553470 4.70 14.88 2.30 26.39 21.51 4.10 19.84 49.60 21.63 22.46 31.13 15.63 41.78 64.01 64.57 0.79 10.21 31.16 93.64 8.33 5.75 0.65 18.59 12.49 53.72 2.00 CsA monotherapy 1.61 2.63 4.72 0.49 kidney transplant recipient 15.09
GSM55347 9.52 19.41 1.02 14.39 30.53 2.09 40.41 50.82 43.94 34.96 47.98 14.55 29.07 45.66 42.86 1.5 12.61 20.67 93.67 7.02 4.72 0.04 28.47 7.32 33.50 4.23 SRL monotherapy 3.79 5.17 4.20 0.4 kidney transplant recipient 38.39
GSM553472 7.84 6.89 0.54 14.90 35.31 8.97 62.69 50.68 27.47 40.76 23.32 35.37 42.47 74.57 43.79 0.44 15.16 20.27 97.44 3.56 1.50 0.08 28.97 2.85 23.38 9.81 CsA monotherapy 1.55 0.9 2.94 0.28 kidney transplant recipient 17.90
GSM553473 8.41 8.89 1.26 25.17 18.40 1.89 29.09 56.07 15.89 37.61 26.57 22.44 42.45 70.69 57.76 1.51 16.50 26.06 93.88 3.92 2.91 0.29 17.58 11.11 42.73 4.42 CsA monotherapy 0.19 2.38 7.23 7.95 kidney transplant recipient 12.57
GSM553474 8.95 9.64 1.62 12.42 34.94 1.33 2.94 41.82 11.23 32.87 27.00 24.39 54.43 60.27 61.13 1.39 21.93 22.50 86.74 12.64 2.67 0.57 35.11 8.79 30.57 6.04 CsA monotherapy 10.52 11.73 6.59 0.13 kidney transplant recipient 7.90
GSM553475 11.82 6.53 1.27 20.85 30.29 2.26 37.49 50.19 19.24 30.53 35.06 27.69 30.07 60.07 52.50 5.92 29.84 17.61 80.19 3.55 2.85 0.32 31.88 11.39 37.90 6.25 CsA monotherapy 1.44 7.7 7.11 4.11 kidney transplant recipient 12.70
GSM553476 17.80 10.47 29.96 0.76 34.67 6.91 39.22 24.48 29.85 11.25 37.24 84.26 1.69 54.83 2.99 1.81 67.69 17.10 89.07 6.82 1.45 17.84 40.58 1.01 24.12 8.14 CsA monotherapy 3.89 5.64 0.32 0.47 kidney transplant recipient 12.43
GSM553477 4.98 18.36 0.67 30.22 32.51 1.88 41.65 50.13 34.03 35.70 31.61 6.34 31.94 53.90 56.91 0.92 3.81 17.47 78.38 5.01 0.14 32.26 6.20 31.12 6.14 SRL monotherapy 6.3 6.96 4.83 0.15 kidney transplant recipient 31.92
GSM553478 2.57 17.52 2.32 8.38 44.32 4.97 10.66 44.62 74.17 29.68 30.31 8.31 13.23 65.65 24.97 1.28 4.21 10.76 94.37 5.21 2.59 0.20 44.42 9.70 23.69 9.65 SRL monotherapy 1.82 2.8 3.82 0.7 kidney transplant recipient 62.89
GSM553479 none Healthy volunteer
GSM553479 none Healthy volunteer
GSM553479 none Healthy volunteer
GSM553479 none Healthy volunteer
GSM553479 none Healthy volunteer
GSM553479 none Healthy volunteer
GSM553479 none Healthy volunteer
GSM553479 none Healthy volunteer
GSM553479 none Healthy volunteer

Tags

  • cell
  • immune system
  • kidney
  • monocyte

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