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Home › Dataset Library › Comparative Transcriptional and Phenotypic Peripheral Blood Analysis of Kidney Recipients under Cyclosporin A or Sirolimus Monotherapy

Dataset: Comparative Transcriptional and Phenotypic Peripheral Blood Analysis of Kidney Recipients under Cyclosporin A or Sirolimus Monotherapy

Due to its low level of nephrotoxicity and capacity to harness tolerogenic pathways, sirolimus (SRL) has been proposed as an alternative...

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Due to its low level of nephrotoxicity and capacity to harness tolerogenic pathways, sirolimus (SRL) has been proposed as an alternative to calcineurin inhibitors in transplantation. The exact mechanisms underlying its unique immunosuppressive profile in humans, however, are still not well understood. In the current study we aimed to depict the in vivo effects of SRL in comparison with cyclosporin A (CSA) by employing gene expression profiling and multiparameter flow cytometry on blood cells collected from stable kidney recipients under immunosuppressant monotherapy. SRL recipients displayed an increased frequency of CD4+CD25highFoxp3+ T cells. However, this was accompanied by an increased number of effector memory T cells and by enrichment in NFkB-related pro-inflammatory expression pathways and monocyte and NK cell lineage-specific transcripts. Furthermore, measurement of a transcriptional signature characteristic of operationally tolerant kidney recipients failed to detect differences between SRL and CSA treated recipients. In conclusion, we show here that the blood transcriptional profile induced by SRL monotherapy in vivo does not resemble that of operationally tolerant recipients and is dominated by innate immune cells and NFkB-related pro-inflammatory events. These data provide novel insights on the complex effects of SLR on the immune system in clinical transplantation. The complete database comprised the expression measurements of 54 675 genes for kidney trasplant recipient PBMC treated with SRL (n=23) and CSA (n=14) monotherapy. 9 healthy controls were also included.

Species:
human

Samples:
46

Source:
E-GEOD-22224

Updated:
Dec.12, 2014

Registered:
Sep.15, 2014


Factors: (via ArrayExpress)
Sample %CD8+CD28- %CD8+CD28+ %CD4 CCR7 - CD45+ %CD8 CD62L+ CD45- %CD3CD4 %CD3-CD56+CD16+ %CD4CD62L+CD45- %CD8CD62L+ CD45+ %CD4 CCR7+ CD45 - %CD3CD8 %CD8CCR7-CD45+ %CD4CD25CD62L %CD4 CCR7+ CD45+ %NKTS %CD8CCR7-CD45 - %CD3DELTA1 %CD8CD62L- CD45+ %CD4 CD62L - CD45- %CD3ALPHABETA DISEASE STATUS %CD4CD8-AB+(DN) %CD4 CD62L-CD45+ %CD4 CD62L+CD45+ %CD19 %CD4 CCR7 – CD45- %CD4FOXP3+ THERAPY %CD3DELTA2 %CD3GAMMADELTA %CD8CD62L- CD45 - %CD8CCR7+ CD45- RATIO DELTA1/DELTA2 %CD8CCR7+ CD45+
GSM553442 20.67 4.28 2.16 70.20 58.23 30.41 44.16 3.84 8.04 30.37 3.64 3.71 8.08 29.16 93.34 0.62 2.03 44.95 92.40 kidney transplant recipient 1.92 0.98 9.91 2.84 81.71 2.78 SRL monotherapy 0.18 1.46 29.93 0.77 3.44 2.25
GSM553443 15.04 9.54 1.01 25.87 48.74 39.21 53.66 5.50 28.73 45.44 6.72 8.71 15.11 1.52 80.54 0.46 4.80 38.88 88.11 kidney transplant recipient 1.92 0.23 7.23 15.90 55.15 10.39 SRL monotherapy 1.46 1.94 63.82 3.42 0.32 9.32
GSM553444 3.45 20.71 4.85 38.29 54.04 29.99 51.99 42.79 17.26 40.46 6.70 6.14 34.52 15.25 53.96 0.8 2.05 10.68 96.76 kidney transplant recipient 4.15 0.10 37.23 8.62 43.37 14.59 SRL monotherapy 1.92 2.47 16.13 2.56 0.42 36.78
GSM553445 22.20 6.07 10.65 13.07 51.52 69.63 35.31 18.75 17.06 44.58 59.28 4.16 13.42 5.42 33.24 1.53 47.27 40.28 94.14 kidney transplant recipient 1.69 8.03 16.38 2.22 58.86 5.60 SRL monotherapy 1.27 3.09 20.89 1.77 1.2 5.71
GSM553446 34.83 8.40 14.13 31.71 68.21 46.24 32.02 31.98 12.81 6.69 20.85 4.27 25.80 17.27 56.95 0.41 10.97 37.25 96.22 kidney transplant recipient 3.08 6.19 24.54 5.37 47.26 3.53 SRL monotherapy 2.22 0.81 25.33 2.63 0.18 19.57
GSM553447 5.04 13.79 0.68 28.01 56.36 2.38 49.95 9.10 30.43 37.60 6.51 6.34 9.90 11.64 84.16 0.27 4.48 41.97 92.93 kidney transplant recipient 2.28 0.04 8.04 6.92 58.99 9.90 SRL monotherapy 6.7 6.78 58.41 3.37 0.04 5.97
GSM553448 4.27 4.27 1.23 19.27 71.81 3.58 43.50 44.04 26.64 22.93 7.89 31.44 4.44 48.62 1.07 4.38 25.39 95.04 kidney transplant recipient 2.94 0.22 30.89 3.58 40.69 4.40 SRL monotherapy 2.19 3.25 30.74 8.99 0.49 34.49
GSM553449 29.41 8.69 6.24 8.22 50.72 37.94 34.73 15.34 17.23 44.51 67.64 6.41 26.88 8.87 27.18 4.48 40.33 35.51 94.30 kidney transplant recipient 1.63 3.30 26.46 1.22 49.65 3.50 SRL monotherapy 0.48 5.14 36.10 0.65 9.33 4.53
GSM553450 21.14 8.72 4.84 5.78 33.60 7.01 18.83 6.36 34.14 52.26 48.90 1.11 59.70 87.18 42.00 3.08 38.11 20.61 95.66 kidney transplant recipient 0.58 48.80 11.76 1.35 1.32 2.69 CsA monotherapy 1.03 3.6 49.75 2.80 2.99 6.30
GSM55345 14.66 4.59 2.26 9.73 66.01 11.13 44.50 20.96 27.13 25.02 71.22 6.83 37.11 6.62 20.3 4.75 56.78 15.87 95.36 kidney transplant recipient 3.13 0.54 39.09 17.90 33.49 4.73 CsA monotherapy 0.45 6.36 13.533 2.6 10.5555555555556 5.84
GSM553452 5.09 16.81 2.23 27.94 61.71 55.46 55.07 12.78 31.64 31.99 14.77 2.19 16.59 9.28 66.22 0.29 12.99 28.64 95.43 kidney transplant recipient 1.64 0.61 15.68 3.55 49.54 3.20 SRL monotherapy 4.11 4.27 46.30 7.03 0.07 11.98
GSM553453 7.92 6.63 0.07 21.47 59.52 34.95 42.02 33.79 81.43 34.10 39.14 4.00 14.87 8.94 50.90 0.3 15.62 47.38 91.42 kidney transplant recipient 11.72 0.33 10.27 5.01 3.63 5.72 SRL monotherapy 2.1 2.9 29.12 3.50 0.14 6.44
GSM553454 10.58 6.22 1.17 9.57 42.26 63.24 47.43 11.60 17.78 46.82 28.37 4.24 2.24 18.27 65.02 1.57 27.92 49.41 86.28 kidney transplant recipient 7.70 0.24 2.92 3.99 78.81 7.38 SRL monotherapy 1.5 7.71 50.91 4.55 1.05 2.06
GSM553455 10.90 12.52 6.92 10.64 62.38 12.71 37.84 32.10 20.56 31.85 38.04 4.45 45.57 11.42 33.84 4.93 29.01 14.06 95.13 kidney transplant recipient 3.06 0.81 47.29 9.65 26.95 3.37 SRL monotherapy 1.63 4.73 28.25 2.09 3.02 26.03
GSM553456 26.66 12.57 8.28 4.62 39.03 48.07 44.20 5.76 26.64 58.22 59.05 10.80 11.39 8.07 37.87 1.93 52.99 36.46 94.08 kidney transplant recipient 1.21 4.67 14.67 4.64 55.69 1.23 CsA monotherapy 0.79 3.69 36.63 0.77 2.44 2.31
GSM553457 34.15 8.45 1.31 7.67 39.27 5.67 35.26 2.61 25.67 53.21 25.13 3.48 11.57 12.48 73.62 3.06 26.45 52.63 94.51 kidney transplant recipient 1.87 0.51 11.60 2.43 61.46 2.06 SRL monotherapy 1.41 4.66 63.27 0.63 2.17 0.62
GSM553458 46.02 7.90 39.50 5.48 32.89 2.39 26.04 74.07 15.27 61.26 90.13 2.30 22.71 5.06 6.88 1.18 17.99 10.40 95.43 kidney transplant recipient 1.34 5.20 58.36 2.42 22.52 3.97 SRL monotherapy 1.86 3.16 2.45 0.30 0.63 2.69
GSM553459 28.92 16.16 1.41 29.07 36.80 48.67 65.95 20.27 17.16 56.37 16.26 2.82 9.48 10.16 82.29 4.51 9.76 16.42 92.41 kidney transplant recipient 1.76 0.09 17.54 0.65 71.96 6.96 CsA monotherapy 1.98 7.51 40.91 0.36 2.28 1.09
GSM553460 12.78 12.73 1.84 16.53 55.60 0.94 36.75 19.34 28.90 39.31 44.86 4.15 46.24 4.06 39.79 4.93 35.99 16.34 76.41 kidney transplant recipient 1.09 45.82 6.02 23.02 3.50 CsA monotherapy 1.68 6.75 28.14 3.90 2.93 11.45
GSM55346 kidney transplant recipient SRL monotherapy
GSM553462 18.14 6.63 1.21 10.07 62.51 56.96 18.26 15.35 11.43 30.98 24.97 7.81 20.48 38.18 62.67 1.19 12.70 59.73 95.78 kidney transplant recipient 0.83 0.33 21.68 3.09 66.88 2.03 CsA monotherapy 2.41 3.45 61.38 1.44 0.49 10.91
GSM553463 11.44 12.25 0.63 8.72 61.36 10.84 53.07 23.55 42.26 33.71 25.78 2.74 12.44 10.24 52.38 0.76 22.36 34.88 82.41 kidney transplant recipient 0.01 12.04 6.11 44.67 4.95 SRL monotherapy 3.6 4.69 45.37 3.56 0.21 18.28
GSM553464 12.07 8.87 21.81 9.99 61.84 3.15 39.90 14.49 12.87 31.65 53.63 4.90 1.17 33.01 43.76 1.89 41.56 38.52 94.76 kidney transplant recipient 4.83 9.68 11.90 6.80 64.15 3.86 CsA monotherapy 2.06 4.1 33.96 0.94 0.92 1.66
GSM553465 10.44 7.71 2.97 10.99 31.77 37.01 38.14 49.23 29.05 59.41 37.78 1.65 48.97 13.17 28.75 4.91 16.49 18.46 85.06 kidney transplant recipient 3.51 0.44 42.96 8.86 19.01 1.65 CsA monotherapy 3.38 7.82 23.30 4.22 1.45 29.25
GSM553466 2.17 9.42 1.33 21.92 80.23 60.71 51.46 48.12 38.64 15.86 16.18 4.00 45.07 1.55 31.74 0.19 8.56 7.56 95.30 kidney transplant recipient 1.09 0.13 40.85 3.84 14.96 3.01 SRL monotherapy 3.63 3.93 21.40 10.07 0.05 42.01
GSM553467 22.24 17.43 0.83 10.36 40.08 5.83 31.98 6.03 15.54 55.69 35.59 15.31 3.03 5.71 60.59 0.33 36.70 64.76 93.73 kidney transplant recipient 2.24 0.42 2.84 9.32 80.60 4.94 SRL monotherapy 1.33 1.97 46.92 0.68 0.25 3.15
GSM553468 1.42 7.55 0.37 29.84 83.89 8.97 58.91 30.57 45.83 14.59 21.81 5.67 29.03 1.97 48.09 0.14 5.79 18.27 96.73 kidney transplant recipient 1.27 0.02 22.80 15.17 24.78 6.66 SRL monotherapy 0.79 1.08 33.80 10.64 0.18 19.46
GSM553469 4.92 10.86 0.71 22.41 68.69 32.00 45.41 42.75 27.49 24.80 12.78 5.32 32.67 2.51 50.11 0.99 6.36 21.60 94.26 kidney transplant recipient 1.42 0.09 32.90 11.05 39.13 8.38 SRL monotherapy 2.92 4.49 28.48 4.82 0.34 32.28
GSM553470 4.70 14.88 2.30 26.39 64.01 19.84 49.60 21.63 22.46 31.13 15.63 4.10 21.51 8.33 64.57 0.79 10.21 31.16 93.64 kidney transplant recipient 5.75 0.65 18.59 12.49 53.72 2.00 CsA monotherapy 1.61 2.63 41.78 4.72 0.49 15.09
GSM55347 9.52 19.41 1.02 14.39 45.66 40.41 50.82 43.94 34.96 47.98 14.55 2.09 30.53 7.02 42.86 1.5 12.61 20.67 93.67 kidney transplant recipient 4.72 0.04 28.47 7.32 33.50 4.23 SRL monotherapy 3.79 5.17 29.07 4.20 0.4 38.39
GSM553472 7.84 6.89 0.54 14.90 74.57 62.69 50.68 27.47 40.76 23.32 35.37 8.97 35.31 3.56 43.79 0.44 15.16 20.27 97.44 kidney transplant recipient 1.50 0.08 28.97 2.85 23.38 9.81 CsA monotherapy 1.55 0.9 42.47 2.94 0.28 17.90
GSM553473 8.41 8.89 1.26 25.17 70.69 29.09 56.07 15.89 37.61 26.57 22.44 1.89 18.40 3.92 57.76 1.51 16.50 26.06 93.88 kidney transplant recipient 2.91 0.29 17.58 11.11 42.73 4.42 CsA monotherapy 0.19 2.38 42.45 7.23 7.95 12.57
GSM553474 8.95 9.64 1.62 12.42 60.27 2.94 41.82 11.23 32.87 27.00 24.39 1.33 34.94 12.64 61.13 1.39 21.93 22.50 86.74 kidney transplant recipient 2.67 0.57 35.11 8.79 30.57 6.04 CsA monotherapy 10.52 11.73 54.43 6.59 0.13 7.90
GSM553475 11.82 6.53 1.27 20.85 60.07 37.49 50.19 19.24 30.53 35.06 27.69 2.26 30.29 3.55 52.50 5.92 29.84 17.61 80.19 kidney transplant recipient 2.85 0.32 31.88 11.39 37.90 6.25 CsA monotherapy 1.44 7.7 30.07 7.11 4.11 12.70
GSM553476 17.80 10.47 29.96 0.76 54.83 39.22 24.48 29.85 11.25 37.24 84.26 6.91 34.67 6.82 2.99 1.81 67.69 17.10 89.07 kidney transplant recipient 1.45 17.84 40.58 1.01 24.12 8.14 CsA monotherapy 3.89 5.64 1.69 0.32 0.47 12.43
GSM553477 4.98 18.36 0.67 30.22 53.90 41.65 50.13 34.03 35.70 31.61 6.34 1.88 32.51 5.01 56.91 0.92 3.81 17.47 78.38 kidney transplant recipient 0.14 32.26 6.20 31.12 6.14 SRL monotherapy 6.3 6.96 31.94 4.83 0.15 31.92
GSM553478 2.57 17.52 2.32 8.38 65.65 10.66 44.62 74.17 29.68 30.31 8.31 4.97 44.32 5.21 24.97 1.28 4.21 10.76 94.37 kidney transplant recipient 2.59 0.20 44.42 9.70 23.69 9.65 SRL monotherapy 1.82 2.8 13.23 3.82 0.7 62.89
GSM553479 Healthy volunteer none
GSM553479 Healthy volunteer none
GSM553479 Healthy volunteer none
GSM553479 Healthy volunteer none
GSM553479 Healthy volunteer none
GSM553479 Healthy volunteer none
GSM553479 Healthy volunteer none
GSM553479 Healthy volunteer none
GSM553479 Healthy volunteer none

Tags

  • cell
  • immune system
  • kidney
  • monocyte

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