{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 8478, "factors": [{"GSM550617": {"GENOTYPE/VARIATION": "Rsk2-KO"}}, {"GSM550617": {"GENOTYPE/VARIATION": "Rsk2-KO"}}, {"GSM550617": {"GENOTYPE/VARIATION": "Rsk2-KO"}}, {"GSM550620": {"GENOTYPE/VARIATION": "wild type"}}, {"GSM550620": {"GENOTYPE/VARIATION": "wild type"}}, {"GSM550620": {"GENOTYPE/VARIATION": "wild type"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 8, "summary_wrapped": "Coffin\u2013Lowry Syndrome (CLS) is a syndromic form of mental retardation caused by loss of function mutations in the X-linked RPS6KA3 gene,...", "pubmed_id": 21116650, "geo_gse_id": "E-GEOD-22137", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["genome", "serine", "syndrome", "threonine"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-profiles-of-the-hippocampus-of-rsk2-ko", "geo_id_plat": "E-GEOD-22137_A-AFFY-36", "name": "Expression profiles of the hippocampus of Rsk2-KO mice", "created": "Nov.24, 2014", "summary": "Coffin\u2013Lowry Syndrome (CLS) is a syndromic form of mental retardation caused by loss of function mutations in the X-linked RPS6KA3 gene, which encodes Rsk2, a serine/threonine kinase involved in spatial memory. We analyzed hippocampal gene expression profiles  in Rsk2-KO mice to identify changes in molecular pathways. Total RNA was extracted from hippocampi from 6 KO and 6 WT (littermates) 2-month-old male mice. For each genotype, equivalent amounts of RNA from 2 mice were pooled and processed for hybridization to the genome wide oligonucleotide microarray (Murine 430A 2.0 Affymetrix, 22.000 probe sets). Thus, 3 independent pooled samples were hybridized for each genotype. We compared hippocampal gene expression profiles from rsk2-KO and normal littermate mice to identify changes in molecular pathways", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22137", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-22137/samples/"}