Dataset: Hydrocortisone induces changes in gene expression and differentiation of immature human enterocytes
It It is known that functional maturation of the small intestine occurring during the weaning period is facilitated by glucocorticoids...
It It is known that functional maturation of the small intestine occurring during the weaning period is facilitated by glucocorticoids (such as hydrocortisone, HC) including the increased expression of digestive hydrolases. However, the molecular mechanism(s) are not well understood, particularly in human gut. Here we report a microarray analysis of HC- induced changes in gene expression in H4 (a human fetal small intestinal epithelial cell line well-characterized in numerous previous studies). This study identified a large number of HC-affected genes, some involved in metabolism, cell cycle regulation, cell polarity, tight junction formation, and interactions with extracellular matrices. These effects could play an important role in HC-mediated enterocyte maturation in vivo and in vitro. hydrocortisone effect on gene expression prlfile in H4 cells over time (no HC, HC_12h, HC_24h, HC_48h, HC_5d and T84). Six samples are analyzed. Control sample is H4 without HC.
- Dec.12, 2014
- Sep.15, 2014