{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 1353, "factors": [{"GSM545849": {"TRANSFECTION": "Myb siRNA"}}, {"GSM545848": {"TRANSFECTION": "non-targeting siRNA"}}, {"GSM545847": {"TRANSFECTION": "mock"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 3, "summary_wrapped": "The c-Myb transcription factor is highly expressed in immature hematopoietic cells and down-regulated during differentiation. To define...", "pubmed_id": 20686118, "geo_gse_id": "E-GEOD-21943", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 3, "tags": ["cell", "macrophage", "monocyte", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "myb-silencing-in-cd14-myeloblasts", "geo_id_plat": "E-GEOD-21943_A-AFFY-33", "name": "MYB silencing in CD14-myeloblasts", "created": "Jun.19, 2014", "summary": "The c-Myb transcription factor is highly expressed in immature hematopoietic cells and down-regulated during differentiation. To define the role of c-Myb during the terminal differentiation of hematopoietic precursors, we studied the effects of its silencing in human primary CD14-myeloblasts, which maintain a granulo-monocyte differentiation bipotentiality. c-Myb-silenced myeloblasts were blocked in the G1 phase of the cell cycle at 24 hours post-nucleofection and subsequently were forced towards macrophage differentiation, as demonstrated by immunophenotypic and morphological analysis. Indeed, c-Myb-silenced CD14- cells differentiate to macrophage even after the treatment with ATRA 10-6 M,  demonstrating that the c-Myb knockdown strongly impairs the ability of myeloblasts to differentiate to granulocytes. Gene expression profiling of c-Myb-silenced CD14- cells identified some potential c-Myb targets that can account for these effects. To maximize siRNA transfection efficiency, we utilized the NucleofectorTM technology (Amaxa). CD14- cells were transfected with a mixture of 3 siRNAs targeting c-Myb mRNA, with a non-targeting siRNA as a negative control (NegCTR) and without siRNA (MOCK). c-Myb siRNAs were transfected at days 1 to 3 after the CD14- myeloblasts purification based on the observation that this timing represented a phase of phisiological increase in c-Myb expression in myeloblasts. Western Blot analysis at 24 hours post-nucleofection demonstrated the c-Myb protein knockdown in MYBsiRNA CD14- cells as compared with control samples (MOCK, NegCTR).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21943", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21943/samples/"}