{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM545567 1": {}}, {"GSM545568 1": {}}, {"GSM545569 1": {}}], "id": 3663, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Although intestinal microbiota play a pivotal role in the development of host immune system this biological issue was not so far studied...", "geo_gse_id": "E-GEOD-21930", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 3, "tags": ["immune system", "point"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-21930_A-AFFY-44", "slug": "expression-data-from-caco-2-cells-co-cultivated-wi", "geo_gds_id": "", "name": "Expression data from Caco-2 cells co-cultivated with B. animalis subsp. lactis BB-12", "created": "Sep.15, 2014", "summary": "Although intestinal microbiota play a pivotal role in the development of host immune system this biological issue was not so far studied in great detail. In this study we examined immune response of Caco-2 enterocytes after incubation with common probiotic Bifidobacterium animalis subsp. lactis BB-12 for 4 hours. We used microarrays to inspect the global gene expression of Caco-2 cells upon co-culturing with B. animalis subsp. lactis BB-12 and several distinct immune-related genes up-regulated during this process. One time point (T4) and two controls (T0) were analysed. T0 represent differentiated Caco-2 cells cultivated for 3 weeks. T4 represents differentiated Caco-2 cells cultivated for 3 weeks plus consequent 4 hours of co-cultivation with B. animalis subsp. lactis BB-12. 3 technical replicates for T0-1, T0-2 or T4 were pooled to a single sample, RNA extracted and further used in gene expression experiments.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21930", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21930/samples/"}