{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM545524": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "spleen", "COMPOUND": "none"}}, {"GSM545524": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "spleen", "COMPOUND": "none"}}, {"GSM545524": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "spleen", "COMPOUND": "none"}}, {"GSM545527": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "C57BL/6", "ORGANISM_PART": "spleen", "COMPOUND": "none"}}, {"GSM545527": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "C57BL/6", "ORGANISM_PART": "spleen", "COMPOUND": "none"}}, {"GSM545527": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "C57BL/6", "ORGANISM_PART": "spleen", "COMPOUND": "none"}}, {"GSM545530": {"DISEASE_STATE": "GM-CSF tumor-bearing", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "spleen", "COMPOUND": "none"}}, {"GSM545530": {"DISEASE_STATE": "GM-CSF tumor-bearing", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "spleen", "COMPOUND": "none"}}, {"GSM545530": {"DISEASE_STATE": "GM-CSF tumor-bearing", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "spleen", "COMPOUND": "none"}}, {"GSM545533": {"DISEASE_STATE": "GM-CSF tumor-bearing", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "tumor infiltrate", "COMPOUND": "none"}}, {"GSM545533": {"DISEASE_STATE": "GM-CSF tumor-bearing", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "tumor infiltrate", "COMPOUND": "none"}}, {"GSM545533": {"DISEASE_STATE": "GM-CSF tumor-bearing", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "tumor infiltrate", "COMPOUND": "none"}}, {"GSM545536": {"DISEASE_STATE": "4T1 tumor-bearing", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "tumor infiltrate", "COMPOUND": "none"}}, {"GSM545536": {"DISEASE_STATE": "4T1 tumor-bearing", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "tumor infiltrate", "COMPOUND": "none"}}, {"GSM545536": {"DISEASE_STATE": "4T1 tumor-bearing", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "tumor infiltrate", "COMPOUND": "none"}}, {"GSM545539": {"DISEASE_STATE": "EL-4 tumor-bearing", "STRAIN_OR_LINE": "C57BL/6", "ORGANISM_PART": "tumor infiltrate", "COMPOUND": "none"}}, {"GSM545539": {"DISEASE_STATE": "EL-4 tumor-bearing", "STRAIN_OR_LINE": "C57BL/6", "ORGANISM_PART": "tumor infiltrate", "COMPOUND": "none"}}, {"GSM545539": {"DISEASE_STATE": "EL-4 tumor-bearing", "STRAIN_OR_LINE": "C57BL/6", "ORGANISM_PART": "tumor infiltrate", "COMPOUND": "none"}}, {"GSM545542": {"DISEASE_STATE": "MCA203 tumor-bearing", "STRAIN_OR_LINE": "C57BL/6", "ORGANISM_PART": "tumor infiltrate", "COMPOUND": "none"}}, {"GSM545542": {"DISEASE_STATE": "MCA203 tumor-bearing", "STRAIN_OR_LINE": "C57BL/6", "ORGANISM_PART": "tumor infiltrate", "COMPOUND": "none"}}, {"GSM545542": {"DISEASE_STATE": "MCA203 tumor-bearing", "STRAIN_OR_LINE": "C57BL/6", "ORGANISM_PART": "tumor infiltrate", "COMPOUND": "none"}}, {"GSM545545": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "bone marrow", "COMPOUND": "none"}}, {"GSM545545": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "bone marrow", "COMPOUND": "none"}}, {"GSM545545": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "bone marrow", "COMPOUND": "none"}}, {"GSM545548": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "bone marrow", "COMPOUND": "In-vitro GM-CSF + IL-6"}}, {"GSM545548": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "bone marrow", "COMPOUND": "In-vitro GM-CSF + IL-6"}}, {"GSM545548": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "bone marrow", "COMPOUND": "In-vitro GM-CSF + IL-6"}}, {"GSM54555": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "bone marrow", "COMPOUND": "In-vitro GM-CSF + G-CSF"}}, {"GSM54555": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "bone marrow", "COMPOUND": "In-vitro GM-CSF + G-CSF"}}, {"GSM54555": {"DISEASE_STATE": "healthy", "STRAIN_OR_LINE": "BALB/c", "ORGANISM_PART": "bone marrow", "COMPOUND": "In-vitro GM-CSF + G-CSF"}}], "id": 6090, "pop_total": 0, "platform": 6, "summary_wrapped": "Tumor growth is associated with a profound alteration of myelopoiesis, leading to recruitment of immunosuppressive cells known as...", "pubmed_id": 20605485, "geo_gse_id": "E-GEOD-21927", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 4, "sample_count": 30, "tags": ["bone", "bone marrow", "cancer", "compartment", "cytokine", "spleen"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-21927_A-AFFY-45", "slug": "transcription-profiling-by-array-of-mus-musculus-m", "geo_gds_id": "", "name": "Transcription profiling by array of Mus musculus myeloid-derived supressor cells from bone marrow or tumor infiltrate", "created": "Nov.11, 2014", "summary": "Tumor growth is associated with a profound alteration of myelopoiesis, leading to recruitment of immunosuppressive cells known as myeloid-derived suppressor cells (MDSCs). Analyzing the cytokines affecting myelo-monocytic differentiation produced by various experimental tumors, we found that  GM-CSF, G-CSF, and IL-6 allowed a rapid generation of MDSCs from precursors present in mouse and human bone marrow (BM). BM-MDSCs induced by GM-CSF+IL-6 possessed the highest tolerogenic activity, as revealed by the ability to impair the priming of IFN- -producing CD8+ T cells upon in vivo adoptive transfer. Moreover, adoptive transfer of syngeneic, GM-CSF+IL-6-conditioned MDSCs to diabetic mice transplanted with allogeneic pancreatic islets resulted in long term acceptance of the allograft and correction of the diabetic status. Cytokines inducing MDSCs acted on a common molecular pathway. Immunoregulatory activity of both tumor-induced and BM-derived MDSCs was entirely dependent on C/EBP  transcription factor, a key component of the emergency myelopoiesis triggered by stress and inflammation. Adoptive transfer of tumor antigen-specific CD8+ T lymphocytes resulted in therapy of established tumors only in mice lacking C/EBP  in myeloid compartment. These data unveil another link between inflammation and cancer and identify a novel molecular target to control tumor-induced immune suppression. We used gene expression analysis to identify those factors, secreted by tumor-infiltrating MDSC, which could drive emathopoiesis. Moreover we compare gene expression profile of tumor-induced MDSC, obtained from either the spleen and the tumor infiltrate of tumor bearing mice,  and in vitro bone marrow-derived MDSC. CD11b+ cells were immunomagnetically enriched from various murine tissue and experimental conditions, and cRNA samples were prepared accordingly to Expression Analysis: Technical Manual. 701021 Rev. 5. Santa Clara, CA, Affymetrix; 2004, and hybridized to the Affymetrix GeneChip MOE430 2.0 array which contains more than 45,000 probe sets, representing more than 34,000 genes. CD11b+ cells obtained from the spleen of healthy BALB/c and C57BL/6 mice were used as reference sample for tumor induced CD11b+ MDSC, enriched from either the spleen and the tumor infiltrate of tumor-bearing mice. Moreover CD11b+ cells enriched from fresh bone marrow were used as reference sample for in vitro bone marrow-differentiated MDSC, obtained with either GM-CSF+IL-6 and GM-CSF+G-CSF 4 days cytokine cocktail treatment.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21927", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21927/samples/"}