ArrayExpress Uploaderarrayexpress_sid6083Media + DMSOFraction4CS (0.07 uM amentoflavone, 0.08 uM quercetin, 0.2 uM chlorogenic acid, and 0.03 uM pseudohypericin)Media + DMSO + LPSFraction + LPS4CS (0.07 uM amentoflavone, 0.08 uM quercetin, 0.2 uM chlorogenic acid, and 0.03 uM pseudohypericin) + LPSMedia + DMSOFraction4CS (0.07 uM amentoflavone, 0.08 uM quercetin, 0.2 uM chlorogenic acid, and 0.03 uM pseudohypericin)Media + DMSO + LPSFraction + LPS4CS (0.07 uM amentoflavone, 0.08 uM quercetin, 0.2 uM chlorogenic acid, and 0.03 uM pseudohypericin) + LPSMedia + DMSOFraction4CS (0.07 uM amentoflavone, 0.08 uM quercetin, 0.2 uM chlorogenic acid, and 0.03 uM pseudohypericin)Media + DMSO + LPSFraction + LPS4CS (0.07 uM amentoflavone, 0.08 uM quercetin, 0.2 uM chlorogenic acid, and 0.03 uM pseudohypericin) + LPS06Hypericum perforatum extracts have been used as dietary supplements to treat conditions including mild-moderate depression and...20303133E-GEOD-21841/profile/8773/arrayexpressuploader118Dec.12, 2014Falseexpression-data-from-lps-stimulated-raw-2647-mouseE-GEOD-21841_A-AFFY-45Expression data from LPS-stimulated RAW 264.7 mouse macrophages treated with Hypericum perforatum fraction and bioactive constituentsNov.11, 2014Hypericum perforatum extracts have been used as dietary supplements to treat conditions including mild-moderate depression and inflammation. A group of four bioactive constituents were identified from an active fraction of the extract. In order to identify the mechanism for the potential anti-inflammatory activity of the identified compounds, we used Affymatrix microarray to study the gene expression profile impacteded by these compounds, as well as the active fraction in LPS-stimulated mouse macrophages. We treated RAW264.7 mouse macrophages with DMSO control, active fraction from Hypericum perforaum extract, and a combination of the 4 putative bioactive compounds, called the 4-component system, all with and without LPS induction. A total of six treatment combinations were included in the final gene expression analysis using microarray.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21841mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21841/samples/