{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM53949": {"GROWTH CONDITION": "cultured  ", "MEDIUM": "serum-containing medium", "CELL TYPE": "parental "}}, {"GSM53949": {"GROWTH CONDITION": "cultured  ", "MEDIUM": "serum-containing medium", "CELL TYPE": "parental "}}, {"GSM53949": {"GROWTH CONDITION": "cultured  ", "MEDIUM": "serum-containing medium", "CELL TYPE": "parental "}}, {"GSM539494": {"GROWTH CONDITION": "cultured  ", "MEDIUM": "stem-cell medium", "CELL TYPE": "spheroid "}}, {"GSM539494": {"GROWTH CONDITION": "cultured  ", "MEDIUM": "stem-cell medium", "CELL TYPE": "spheroid "}}, {"GSM539494": {"GROWTH CONDITION": "cultured  ", "MEDIUM": "stem-cell medium", "CELL TYPE": "spheroid "}}, {"GSM539497": {"GROWTH CONDITION": "xenografted  ", "MEDIUM": "serum-containing medium", "CELL TYPE": "parental "}}, {"GSM539497": {"GROWTH CONDITION": "xenografted  ", "MEDIUM": "serum-containing medium", "CELL TYPE": "parental "}}, {"GSM539497": {"GROWTH CONDITION": "xenografted  ", "MEDIUM": "serum-containing medium", "CELL TYPE": "parental "}}, {"GSM539500": {"GROWTH CONDITION": "xenografted  ", "MEDIUM": "stem-cell medium", "CELL TYPE": "spheroid "}}, {"GSM539500": {"GROWTH CONDITION": "xenografted  ", "MEDIUM": "stem-cell medium", "CELL TYPE": "spheroid "}}, {"GSM539500": {"GROWTH CONDITION": "xenografted  ", "MEDIUM": "stem-cell medium", "CELL TYPE": "spheroid "}}], "id": 3651, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Current approaches to the preclinical investigation for novel cancer therapies rely heavily on the use of traditional cancer cell lines...", "geo_gse_id": "E-GEOD-21612", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 12, "tags": ["cancer", "cell", "compartment", "line", "lung", "lung cancer", "serum", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-21612_A-AFFY-44", "slug": "transcription-profiling-by-array-of-nci-h1299-pare", "geo_gds_id": "", "name": "Transcription profiling by array of NCI-H1299 parental and spheroid cells", "created": "Sep.15, 2014", "summary": "Current approaches to the preclinical investigation for novel cancer therapies rely heavily on the use of traditional cancer cell lines maintained in serum-containing conditions. The discrepancy between promising preclinical efficacy and clinical outcome of most novel anticancer agents emphasizes a need for developing predictive preclinical models that preserve the integrity of original patient tumors, including cancer stem cell (CSC) compartment. In this study, we isolate and characterize CSCs from a non-small cell lung cancer cell line, NCI-H1299, by selectively propagating the cells in a stem-cell culture condition. Isolated CSCs proliferated as nonadherent spheroids, displayed capacity to differentiate and self-renew and exhibited higher tumorigenic potential compared to the parental cells. The gene expression profiles of NCI-H1299 parental cells (serum-containing medium), CSCs (stem-cell medium), and xenograft tumor derived from both cell types were studied by Affymetrix array analysis", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21612", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21612/samples/"}