{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM539070": {"AGE": "Day 4", "CELL TYPE": "ST2 adipocytes", "STRAIN OR LINE": "BC8"}}, {"GSM539070": {"AGE": "Day 4", "CELL TYPE": "ST2 adipocytes", "STRAIN OR LINE": "BC8"}}, {"GSM539070": {"AGE": "Day 4", "CELL TYPE": "ST2 adipocytes", "STRAIN OR LINE": "BC8"}}, {"GSM539070": {"AGE": "Day 4", "CELL TYPE": "ST2 adipocytes", "STRAIN OR LINE": "BC8"}}, {"GSM539070": {"AGE": "Day 4", "CELL TYPE": "ST2 adipocytes", "STRAIN OR LINE": "BC8"}}, {"GSM539070": {"AGE": "Day 4", "CELL TYPE": "ST2 adipocytes", "STRAIN OR LINE": "BC8"}}, {"GSM539076": {"AGE": "Day 8", "CELL TYPE": "3T3-L1 adipocytes", "STRAIN OR LINE": "Swiss albino"}}, {"GSM539076": {"AGE": "Day 8", "CELL TYPE": "3T3-L1 adipocytes", "STRAIN OR LINE": "Swiss albino"}}, {"GSM539076": {"AGE": "Day 8", "CELL TYPE": "3T3-L1 adipocytes", "STRAIN OR LINE": "Swiss albino"}}, {"GSM539076": {"AGE": "Day 8", "CELL TYPE": "3T3-L1 adipocytes", "STRAIN OR LINE": "Swiss albino"}}, {"GSM539076": {"AGE": "Day 8", "CELL TYPE": "3T3-L1 adipocytes", "STRAIN OR LINE": "Swiss albino"}}, {"GSM539076": {"AGE": "Day 8", "CELL TYPE": "3T3-L1 adipocytes", "STRAIN OR LINE": "Swiss albino"}}], "id": 6061, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "PPAR\u03b3 is a master transcriptional regulator of adipogenesis. Hence, the identification of PPAR\u03b3 coactivators should help reveal...", "geo_gse_id": "E-GEOD-21594", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 12, "tags": ["adipocyte", "adipose tissue", "cell", "genome", "glucose", "insulin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "multiple-roles-for-steroid-receptor-rna-activator", "geo_id_plat": "E-GEOD-21594_A-AFFY-45", "name": "Multiple Roles for Steroid Receptor RNA Activator (SRA) in Regulation of Adipogenesis and Insulin Sensitivity", "created": "Nov.11, 2014", "summary": "PPAR\u03b3 is a master transcriptional regulator of adipogenesis. Hence, the identification of PPAR\u03b3 coactivators should help reveal mechanisms controlling gene expression in adipose tissue development and physiology. We show that the non-coding RNA Steroid receptor RNA Activator, SRA, associates with PPAR\u03b3 and coactivates PPAR\u03b3-dependent reporter gene expression. Overexpression of SRA in ST2 adipocyte precursor cells promotes their differentiation into adipocytes. Conversely, knockdown of endogenous SRA inhibits 3T3-L1 preadipocyte differentiation.  Microarray analysis reveals hundreds of SRA-responsive genes in adipocytes, including genes in cell cycle, insulin and TNF\u03b1 signaling pathways. Some functions of SRA may involve mechanisms other than coactivation of PPAR\u03b3. SRA increases insulin-stimulated glucose uptake in adipocytes. SRA promotes S-phase entry during mitotic clonal expansion, decreases expression of cyclin-dependent kinase inhibiters p21Cip1 and p27Kip1, and increases phosphorylation of Cdk1/Cdc2. SRA also inhibits the TNF\u03b1-induced phosphorylation of c-Jun NH2-terminal kinase. In conclusion, SRA enhances adipogenesis and adipocyte function through multiple pathways. Total RNA was isolated from fully differentiated (MDIT day 4) SRA overexpressing (pMSCV-SRA) and control (pMSCV empty vector) ST2 adipocytes, or fully differentiated (MDIT day 8) shSRA knockdown (pSuperior-shSRA) or shControl (pSuperior-shcontrol) 3T3-L1 adipocytes. Genome wide gene expression analysis was performed using Affymetrix mouse genome 430 2.0 arrays. Triplicate samples were analyzed.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21594", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21594/samples/"}