{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM532558": {"AGENT": "mock-transfected"}}, {"GSM532559": {"AGENT": "RECK-overexpressing"}}, {"GSM532560": {"AGENT": "RECK/4NQ-overexpressing (in which four N-glycosylation asparagine residues of RECK were replaced with glutamine residues)"}}], "id": 3633, "pop_total": 0, "platform": 4, "summary_wrapped": "RECK, a glycosylphosphatidylinositol-anchored glycoprotein, inhibits the enzymatic activities of some matrix metalloproteinases (MMP),...", "geo_gse_id": "E-GEOD-21306", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 3, "tags": ["cell", "glutamine", "glycoprotein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-21306_A-AFFY-44", "slug": "expression-data-from-reck-overexpressing-ht1080-fi", "geo_gds_id": "", "name": "Expression data from RECK-overexpressing HT1080 fibrosarcoma cells", "created": "Sep.15, 2014", "summary": "RECK, a glycosylphosphatidylinositol-anchored glycoprotein, inhibits the enzymatic activities of some matrix metalloproteinases (MMP), thereby suppressing tumor cell metastasis; however, the detailed mechanism is still obscure. In this study, we compared the gene expression profiles between mock- and RECK-transfected HT1080 cells. Three established cell lines were selected for RNA extraction and hybridization on Affymetrix microarrays: (1) mock-transfected HT1080 cells, designated as HT1080-Zeo, (2) RECK-overexpressing HT1080 cells, designated as HT1080-RECK, and (3) RECK/4NQ-overexpressing HT1080 cells (in which four N-glycosylation asparagine residues of RECK (Asn86, Asn200, Asn297, and Asn352) were replaced with glutamine residues), designated as HT1080-RECK/4NQ.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21306", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21306/samples/"}