{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM529590": {"GENOTYPE": "CBFb-SMMHC chimera"}}, {"GSM529590": {"GENOTYPE": "CBFb-SMMHC chimera"}}, {"GSM529590": {"GENOTYPE": "CBFb-SMMHC chimera"}}, {"GSM529590": {"GENOTYPE": "CBFb-SMMHC chimera"}}, {"GSM529594": {"GENOTYPE": "CBFb-SMMHCd179-221 chimera"}}, {"GSM529594": {"GENOTYPE": "CBFb-SMMHCd179-221 chimera"}}, {"GSM529594": {"GENOTYPE": "CBFb-SMMHCd179-221 chimera"}}, {"GSM529594": {"GENOTYPE": "CBFb-SMMHCd179-221 chimera"}}], "id": 6031, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Dominant RUNX1 inhibition has been proposed as a common pathway for CBF-leukemia.  CBFb-SMMHC, a fusion protein in human acute myeloid...", "geo_gse_id": "E-GEOD-21155", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 8, "tags": ["acute myeloid leukemia", "leukemia", "myeloid leukemia", "protein", "spleen"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "accelerated-leukemogenesis-by-truncated-cbfb-smmhc", "geo_id_plat": "E-GEOD-21155_A-AFFY-45", "name": "Accelerated leukemogenesis by truncated CBFb-SMMHC defective in high-affinity binding with RUNX1", "created": "Nov.11, 2014", "summary": "Dominant RUNX1 inhibition has been proposed as a common pathway for CBF-leukemia.  CBFb-SMMHC, a fusion protein in human acute myeloid leukemia (AML), dominantly inhibits RUNX1 largely through its RUNX1 high-affinity binding domain (HABD). We generated knock-in mice expressing CBFb-SMMHC with a HABD deletion, CBFb-SMMHCd179-221. These mice developed leukemia highly efficiently, even though hematopoietic defects associated with Runx1-inhibition were partially rescued. To identify changes in gene expression with the deletion of the HABD, we compared the gene expression profile in leukemia samples from mice expressing CBFb-SMMHCd179-221 with those from mice expressing full length CBFb-SMMHC. Spleen cells were isolated from leukemic knock-in mice with full length CBFb-SMMHC at 2 months after ENU treatment and 2 leukemic CBFb-SMMHCd179-221 expressing chimeric mice at 3 weeks after birth. For each genotype, we performed two independent experiments with 4 Affymetrix GeneChip 430 chips.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21155", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21155/samples/"}