{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM529453": {"CELL TYPE": "CD8a+ SPDCs"}}, {"GSM529453": {"CELL TYPE": "CD8a+ SPDCs"}}, {"GSM529455": {"CELL TYPE": "CD8a- SPDCs"}}, {"GSM529455": {"CELL TYPE": "CD8a- SPDCs"}}], "id": 6030, "pop_total": 0, "platform": 6, "summary_wrapped": "Microarray analysis of CD8\u03b1+ SPDCs and CD8\u03b1- SPDCs from C57BL/6 mice; to elucidate genes specifically expressed in CD8\u03b1+ SPDCs CD8\u03b1+ DCs...", "geo_gse_id": "E-GEOD-21144", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["genome", "spleen"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-in-cd8-spdcs-and-cd8-spdcs", "geo_id_plat": "E-GEOD-21144_A-AFFY-45", "name": "Gene expression in CD8\u03b1+ SPDCs and CD8\u03b1- SPDCs", "created": "Nov.11, 2014", "summary": "Microarray analysis of CD8\u03b1+ SPDCs and CD8\u03b1- SPDCs from C57BL/6 mice; to elucidate genes specifically expressed in CD8\u03b1+ SPDCs CD8\u03b1+ DCs and CD8\u03b1- DCs were isolated from the spleen of C57BL/6 mice. Total RNA was extracted with an RNeasy kit (Qiagen), and purified using an Oligotex mRNA Kit (Pharmacia). Fragmented and biotin-labeled cDNA was synthesized from 100 ng purified mRNA using the Ovation Biotin System (Nugen), according to the manufacturer\u2019s protocol. cDNA was hybridized to Affymetrix Murine Genome 430 2.0 microarray chips (Affymetrix) according to the manufacturer\u2019s instructions. Hybridized chips were stained, washed and scanned using a GeneArray Scanner (Affymetrix). Data analysis was performed with Microarray Suite software (Version 5.0, Affymetrix) and GeneSpring software (Silicon Genetics).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21144", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21144/samples/"}