{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6024, "factors": [{"GSM526693": {"TIME STIMULATED WITH ANTI-IGM": "unstimulated"}}, {"GSM526694": {"TIME STIMULATED WITH ANTI-IGM": "3h"}}, {"GSM526695": {"TIME STIMULATED WITH ANTI-IGM": "8h"}}, {"GSM526696": {"TIME STIMULATED WITH ANTI-IGM": "16h"}}, {"GSM526693": {"TIME STIMULATED WITH ANTI-IGM": "unstimulated"}}, {"GSM526694": {"TIME STIMULATED WITH ANTI-IGM": "3h"}}, {"GSM526695": {"TIME STIMULATED WITH ANTI-IGM": "8h"}}, {"GSM526696": {"TIME STIMULATED WITH ANTI-IGM": "16h"}}, {"GSM526693": {"TIME STIMULATED WITH ANTI-IGM": "unstimulated"}}, {"GSM526694": {"TIME STIMULATED WITH ANTI-IGM": "3h"}}, {"GSM526695": {"TIME STIMULATED WITH ANTI-IGM": "8h"}}, {"GSM526696": {"TIME STIMULATED WITH ANTI-IGM": "16h"}}, {"GSM526693": {"TIME STIMULATED WITH ANTI-IGM": "unstimulated"}}, {"GSM526694": {"TIME STIMULATED WITH ANTI-IGM": "3h"}}, {"GSM526695": {"TIME STIMULATED WITH ANTI-IGM": "8h"}}, {"GSM526696": {"TIME STIMULATED WITH ANTI-IGM": "16h"}}, {"GSM526693": {"TIME STIMULATED WITH ANTI-IGM": "unstimulated"}}, {"GSM526694": {"TIME STIMULATED WITH ANTI-IGM": "3h"}}, {"GSM526695": {"TIME STIMULATED WITH ANTI-IGM": "8h"}}, {"GSM526696": {"TIME STIMULATED WITH ANTI-IGM": "16h"}}, {"GSM526693": {"TIME STIMULATED WITH ANTI-IGM": "unstimulated"}}, {"GSM526694": {"TIME STIMULATED WITH ANTI-IGM": "3h"}}, {"GSM526695": {"TIME STIMULATED WITH ANTI-IGM": "8h"}}, {"GSM526696": {"TIME STIMULATED WITH ANTI-IGM": "16h"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Triggering of B cell receptors (BCR) induces a massive synthesis of NFATc1 in splenic B cells. By inactivating the Nfatc1 gene and re-...", "pubmed_id": 21464221, "geo_gse_id": "E-GEOD-21063", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 24, "tags": ["cell", "class", "encephalomyelitis", "immunoglobulin"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "nfatc1-controls-the-survival-function-and-suppress", "geo_id_plat": "E-GEOD-21063_A-AFFY-45", "name": "NFATc1 controls the survival, function and suppressive capacity of B lymphocytes upon B cell receptor stimulation", "created": "Nov.11, 2014", "summary": "Triggering of B cell receptors (BCR) induces a massive synthesis of NFATc1 in splenic B cells. By inactivating the Nfatc1 gene and re-expressing NFATc1 we show that NFATc1 levels are critical for the survival of splenic B cells upon BCR stimulation. NFATc1 ablation led to decreased BCR-induced Ca++ flux and proliferation of splenic B cells, increased apoptosis and suppressed germinal centre formation and immunoglobulin class switch by T cell-independent antigens. By controlling IL-10 synthesis in B cells, NFATc1 supported the proliferation and IL-2 synthesis of T cells in vitro and appeared to contribute to the mild clinical course of Experimental Autoimmune Encephalomyelitis in mice bearing NFATc1-/- B cells. These data indicate NFATc1 as a key factor controlling B cell function. Splenic mice cells were isolated from mice bearing NFATc1 deficient B-cells and from control mice, stimulated with anti-IgM for 0h, 3h, 8h and 16h, respectively and isolated using Milteny beads to enrich the B cell population. This experiment was performed in 3 biological replicates.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21063", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-21063/samples/"}