{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM52052": {"CELL TYPE": "stem cells G48"}}, {"GSM52052": {"CELL TYPE": "stem cells G48"}}, {"GSM520523": {"CELL TYPE": "stem cells G52"}}, {"GSM520523": {"CELL TYPE": "stem cells G52"}}, {"GSM520525": {"CELL TYPE": "stem cells G63"}}, {"GSM520525": {"CELL TYPE": "stem cells G63"}}], "id": 3605, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Glioblastoma multiforme is one of the most devastating cancers and presents unique challenges to therapy due to its aggressive behaviour....", "geo_gse_id": "E-GEOD-20736", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["brain", "cancer", "cell", "glioblastoma multiforme", "intermediate"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-20736_A-AFFY-44", "slug": "microarray-analysis-of-differentiation-of-human-2", "geo_gds_id": "", "name": "Microarray analysis of differentiation of human glioblastoma stem cells", "created": "Sep.15, 2014", "summary": "Glioblastoma multiforme is one of the most devastating cancers and presents unique challenges to therapy due to its aggressive behaviour. Cancer stem cells have been described to be the only cell population with tumorogenic capacity in glioblastoma. Therefore, effective therapeutic strategies targeting these cells may be beneficial. We have established different cultures of glioblastoma stem cells (GSCs) derived from surgical specimens and found that, after induction of differentiation, NF\u03baB was activated, which allows intermediate tumor precursor cells to remain cycling. We also showed that blockade of NF\u03baB signaling in differentiating GSCs by different genetic strategies or treatment with small molecule inhibitors, promoted replication arrest, progression to a mature phenotype, mainly neuronal cells, and senescence. This effect was partly mediated by downregulation of the NF\u03baB target gene cyclin D1. Furthermore, intravenous treatment of immunodeficient mice bearing human GSC-derived tumors with a novel small-molecule inhibitor of the NF\u03baB pathway induced senescence of tumor cells but no ultraestructural alterations of the brain parenchymal cells were detected. These findings reveal that activation of NF\u03baB may keep differentiating GSCs from acquiring a mature postmitotic phenotype, thus allowing cell proliferation, and support the rationale for therapeutic strategies aimed at promoting premature senescence in GSCs undergoing differentiation. Gene expression in differentiated cells relative to stem cells in three different glioblastoma cultures", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20736", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20736/samples/"}