{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 6005, "factors": [{"GSM519105": {"STIMULATED": "yes", "GENOTYPE": "Stat5a/b knockout"}}, {"GSM519106": {"STIMULATED": "yes", "GENOTYPE": "WT"}}, {"GSM519105": {"STIMULATED": "yes", "GENOTYPE": "Stat5a/b knockout"}}, {"GSM519106": {"STIMULATED": "yes", "GENOTYPE": "WT"}}, {"GSM519105": {"STIMULATED": "yes", "GENOTYPE": "Stat5a/b knockout"}}, {"GSM519106": {"STIMULATED": "yes", "GENOTYPE": "WT"}}, {"GSM519": {"STIMULATED": "no", "GENOTYPE": "Stat5a/b knockout"}}, {"GSM519112": {"STIMULATED": "no", "GENOTYPE": "WT"}}, {"GSM519": {"STIMULATED": "no", "GENOTYPE": "Stat5a/b knockout"}}, {"GSM519112": {"STIMULATED": "no", "GENOTYPE": "WT"}}, {"GSM519": {"STIMULATED": "no", "GENOTYPE": "Stat5a/b knockout"}}, {"GSM519112": {"STIMULATED": "no", "GENOTYPE": "WT"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Cytokines control the biology of hematopoietic stem and progenitor cells in part through the transcription factors STAT5a/b.  CCN3/NOV...", "pubmed_id": 20720003, "geo_gse_id": "E-GEOD-20684", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 12, "tags": ["gas"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "the-gene-encoding-the-hematopoietic-stem-cell-regu", "geo_id_plat": "E-GEOD-20684_A-AFFY-45", "name": "The gene encoding the hematopoietic stem cell regulator CCN3 (NOV) is under direct cytokine control through the transcription factors STAT5a/b.", "created": "Nov.11, 2014", "summary": "Cytokines control the biology of hematopoietic stem and progenitor cells in part through the transcription factors STAT5a/b.  CCN3/NOV has been reported as a positive regulator of hematopoietic stem and progenitor cells.  We report microarray analyses of Lineage- Sca-1+ c-Kit+ (KSL) cells in the presence and absence of STAT5a/b.  Expression of the ccn3 gene was induced over 100-fold in control, but not STAT5a/b-null cells, upon stimulation with a cocktail containing IL-3, IL-6, SCF, TPO and Flt3 ligand.  Among the cytokines, IL-3 elevated ccn3 mRNA level in Lineage- c-Kit+ (KL) cells and 32D cells.  ChIP assays using 32D cells revealed IL-3-induced binding of STAT5a/b to a GAS site in the ccn3 gene promoter.  This is the first report to link two molecules with importance in the regulation of HSCs, CCN3 and STAT5a/b.  We report that the regulation and expression of the ccn3 gene is directly controlled by IL-3 through the transcription factors STAT5a/b. Six Control and Six Stat5a/b-null KSL cells, including three biological replications, were unstimulated or stimulated with a cocktail containing IL-3, IL-6, SCF, TPO and FL.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20684", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20684/samples/"}