ArrayExpress Uploader08465MMTV-PyMTMMTV-PyMTMMTV-PyMTMMTV-PyMTMMTV-PyMTMMTV-tva RCAS-PyMTMMTV-tva RCAS-PyMTMMTV-tva RCAS-PyMTMMTV-tva RCAS-PyMTMMTV-tva RCAS-PyMTK6-tva RCAS-PyMTK6-tva RCAS-PyMTK6-tva RCAS-PyMTK6-tva RCAS-PyMTK6-tva RCAS-PyMTarrayexpress_sid8Conventional transgenic and knockout models do not allow selective introduction of oncogenic alterations into the progenitor population...21532625/profile/8773/arrayexpressuploader115avian leukosisbreastbreast cancercancercellkeratinlinemiddleDec.12, 2014FalseE-GEOD-20614_A-AFFY-36gene-expression-profiling-of-mouse-tumors-arisinghttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20614Gene expression profiling of mouse tumors arising from Keratin 6-positive mammary cellsNov.24, 2014Conventional transgenic and knockout models do not allow selective introduction of oncogenic alterations into the progenitor population of mammary cells; thus, the role of progenitor cells in mammary tumorigenesis is yet unknown. By generating transgenic mice expressing tva – encoding the receptor for avian leukosis virus subgroup A (ALV/A) – from the Keratin 6a (K6) gene promoter, we found that K6+ mammary cells are bipotential progenitor cells, but not stem cells. These K6+ cells were readily induced to form tumors by intraductal injection of RCAS (an ALV/A-derived vector) carrying the gene encoding polyoma middle T antigen. Compared with tumors induced by the same oncogene-expressing virus in transgenic mice expressing tva from the commonly used MMTV LTR or other murine models of breast cancer, tumors in this K6-tva line were unique in that they resemble the normal breast-like subtype of human breast cancer. Consequently, these observations suggest that the cell of origin affects mammary tumor phenotypes. This K6-tva model may be useful for preclinical testing of targeted therapy for normal-like breast cancers in patients. Keywords: Three group comparison We carried out Affymetrix array analysis of five RCAS-PyMT-induced tumors each from K6-tva mice and MMTV-tva mice, as well as five mammary tumors from MMTV-PyMT transgenic mice.E-GEOD-20614mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20614/samples/