{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 8465, "factors": [{"GSM517882": {"TRANSGENIC": "MMTV-PyMT"}}, {"GSM517882": {"TRANSGENIC": "MMTV-PyMT"}}, {"GSM517882": {"TRANSGENIC": "MMTV-PyMT"}}, {"GSM517882": {"TRANSGENIC": "MMTV-PyMT"}}, {"GSM517882": {"TRANSGENIC": "MMTV-PyMT"}}, {"GSM517887": {"TRANSGENIC": "MMTV-tva RCAS-PyMT"}}, {"GSM517887": {"TRANSGENIC": "MMTV-tva RCAS-PyMT"}}, {"GSM517887": {"TRANSGENIC": "MMTV-tva RCAS-PyMT"}}, {"GSM517887": {"TRANSGENIC": "MMTV-tva RCAS-PyMT"}}, {"GSM517887": {"TRANSGENIC": "MMTV-tva RCAS-PyMT"}}, {"GSM517892": {"TRANSGENIC": "K6-tva RCAS-PyMT"}}, {"GSM517892": {"TRANSGENIC": "K6-tva RCAS-PyMT"}}, {"GSM517892": {"TRANSGENIC": "K6-tva RCAS-PyMT"}}, {"GSM517892": {"TRANSGENIC": "K6-tva RCAS-PyMT"}}, {"GSM517892": {"TRANSGENIC": "K6-tva RCAS-PyMT"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 8, "summary_wrapped": "Conventional transgenic and knockout models do not allow selective introduction of oncogenic alterations into the progenitor population...", "pubmed_id": 21532625, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 15, "tags": ["avian leukosis", "breast", "breast cancer", "cancer", "cell", "keratin", "line", "middle"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-20614_A-AFFY-36", "slug": "gene-expression-profiling-of-mouse-tumors-arising", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20614", "geo_gds_id": "", "name": "Gene expression profiling of mouse tumors arising from Keratin 6-positive mammary cells", "created": "Nov.24, 2014", "summary": "Conventional transgenic and knockout models do not allow selective introduction of oncogenic alterations into the progenitor population of mammary cells; thus, the role of progenitor cells in mammary tumorigenesis is yet unknown. By generating transgenic mice expressing tva \u2013 encoding the receptor for avian leukosis virus subgroup A (ALV/A) \u2013 from the Keratin 6a (K6) gene promoter,  we found that K6+ mammary cells are bipotential progenitor cells, but not stem cells.  These K6+ cells were readily induced to form tumors by intraductal injection of RCAS (an ALV/A-derived vector) carrying the gene encoding polyoma middle T antigen.  Compared with tumors induced by the same oncogene-expressing virus in transgenic mice expressing tva from the commonly used MMTV LTR or other murine models of breast cancer, tumors in this K6-tva line were unique in that they resemble the normal breast-like subtype of human breast cancer.  Consequently, these observations suggest that the cell of origin affects mammary tumor phenotypes.  This K6-tva model may be useful for preclinical testing of targeted therapy for normal-like breast cancers in patients.  Keywords: Three group comparison We carried out Affymetrix array analysis of five RCAS-PyMT-induced tumors each from K6-tva mice and MMTV-tva mice, as well as five mammary tumors from MMTV-PyMT transgenic mice.", "geo_gse_id": "E-GEOD-20614", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20614/samples/"}