{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 1324, "factors": [{"GSM509114": {"AGE": "85", "DISEASE STATUS": "healthy control"}}, {"GSM509113": {"AGE": "79", "DISEASE STATUS": "parkinson's disease"}}, {"GSM509112": {"AGE": "86", "DISEASE STATUS": "healthy control"}}, {"GSM509": {"AGE": "85", "DISEASE STATUS": "parkinson's disease"}}, {"GSM509110": {"AGE": "81", "DISEASE STATUS": "healthy control"}}, {"GSM509112": {"AGE": "86", "DISEASE STATUS": "healthy control"}}, {"GSM509108": {"AGE": "84", "DISEASE STATUS": "parkinson's disease"}}, {"GSM509": {"AGE": "85", "DISEASE STATUS": "parkinson's disease"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 3, "summary_wrapped": "Analysis of substantia nigra from postmortem brains of 4 patients with Parkinson\u2019s disease (PD). Results provide insight into the...", "pubmed_id": 20926834, "geo_gse_id": "E-GEOD-20314", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 8, "tags": ["brain", "disease", "substantia nigra"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "systematic-meta-analysis-and-replication-of-genome", "geo_id_plat": "E-GEOD-20314_A-AFFY-33", "name": "Systematic meta-analysis and replication of genome-wide expression studies of Parkinson\u2019s disease: 4", "created": "Jun.19, 2014", "summary": "Analysis of substantia nigra from postmortem brains of 4 patients with Parkinson\u2019s disease (PD). Results provide insight into the molecular processes perturbed in the PD substantia nigra. Fresh frozen tissue blocks from cerebellar hemispheres of PD patients and controls were provided by the Queens Square Brain Bank for Neurological Disorders (London, UK). Total RNA was isolated from about 500 mg fresh frozen postmortem tissue from each subject by using Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol and further purified using the RNeasy mini kit (Qiagen, Hilden, Germany). Quality of the extracted total RNA was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Boblingen, Germany). Cerebellar mRNA with the following criteria: two distinct visible peaks for 18 s and 20 s RNA with lack of multiple small peaks indicating major degradation in Agilent Bioanalyzer fluorograms and GAPDH 3\u2032/5\u2032 ratios smaller 5.0 and percentage present calls greater 40% for the parameter assessed by chip hybridization were hybridized on Affymetrix human U133A GeneChip Array. Sample labeling, hybridization to arrays and image scanning (Affymetrix\u00ae GeneChip\u00ae Scanner 2500, Agilent technologies, Boblingen, Germany) were performed using standard Affymetrix\u00ae protocol (Affymetrix\u00ae Expression Analysis Technical Manual) as described previously (Bonin et al,  Brain Res. Mol. Brain Res. 126, 2004). The Affymetrix .CEL files were normalized to \u201call probe sets\u201d in a standardized matter, and scaled to 100 by the MAS5 algorithm implemented in the Bioconductor package.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20314", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20314/samples/"}