{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM505624": {"SPHINGOSINE KINASE 1 ACTIVITY": "  ", "TNFA ACTIVITY": "over-expression"}}, {"GSM505624": {"SPHINGOSINE KINASE 1 ACTIVITY": "  ", "TNFA ACTIVITY": "over-expression"}}, {"GSM505626": {"SPHINGOSINE KINASE 1 ACTIVITY": "normal", "TNFA ACTIVITY": "normal"}}, {"GSM505624": {"SPHINGOSINE KINASE 1 ACTIVITY": "  ", "TNFA ACTIVITY": "over-expression"}}, {"GSM505626": {"SPHINGOSINE KINASE 1 ACTIVITY": "normal", "TNFA ACTIVITY": "normal"}}, {"GSM505629": {"SPHINGOSINE KINASE 1 ACTIVITY": "normal", "TNFA ACTIVITY": "over-expression"}}, {"GSM505629": {"SPHINGOSINE KINASE 1 ACTIVITY": "normal", "TNFA ACTIVITY": "over-expression"}}, {"GSM505629": {"SPHINGOSINE KINASE 1 ACTIVITY": "normal", "TNFA ACTIVITY": "over-expression"}}], "id": 5968, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "The study analyzes analyzes gene expression changes in the ankle joint in mouse TNFa overexpression models with or without sphingosine...", "geo_gse_id": "E-GEOD-20152", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 8, "tags": ["ankle", "ankle joint", "arthritis", "bone", "joint", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "the-role-of-sphk1-in-htnf-induced-inflammation", "geo_id_plat": "E-GEOD-20152_A-AFFY-45", "name": "The role of SphK1 in hTNF\u03b1 induced inflammation", "created": "Nov.11, 2014", "summary": "The study analyzes analyzes gene expression changes in the ankle joint in mouse TNFa overexpression models with or without sphingosine kinase 1 activity. SphK1 is a sphingolipid enzyme that converts sphingosine to bioactive sphingosine-1-phosphate (S1P).  Recent data suggest a potential relationship between SphK1 and TNF\u03b1 and have implicated SphK1/S1P in the development and progression of inflammation.  Here we further study the relationship of TNF\u03b1 and SphK1 using an in vivo model.  Transgenic hTNF\u03b1 mice, which develop a spontaneous arthritis (limited to paws) at 20 weeks, were crossed with SphK1 activity null mice (SphK1-/-) to study the development of inflammatory arthritis in the functional absence of SphK1.  Results show that hTNF/SphK1-/- have significantly less severity and progression of arthritis and bone erosions as measured through micro-CT images.  Additionally, less COX-2 protein, mTNF\u03b1 transcript levels and fewer Th 17 cells were detected in the joints of hTNF/SphK1-/- compared to hTNF/SphK1+/+ mice.  Microarray analysis of the ankle joint showed that hTNF/SphK1-/- mice have increased transcript levels of IL-6 and SOCS3 compared to hTNF/SphK1+/+ mice.  Finally, fewer mature osteoclasts were detected in the ankle joints of hTNF/SphK1-/- mice compared to hTNF/SphK1+/+ mice.  These data show that SphK1 plays a role in hTNF\u03b1 induced inflammatory arthritis, potentially through a novel pathway involving IL-6 and SOCS3. Two wild-type replicates; three replicates of human TNFa transgene overexpression and normal sphingosine kinase 1; three replicates of human TNFa transgene overexpression and sphingosine kinase 1 null.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20152", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20152/samples/"}