ArrayExpress Uploaderarrayexpress_sidhumanundifferentiatedHuman Embryonic Stem CellsundifferentiatedHuman Embryonic Stem CellsundifferentiatedHuman Embryonic Stem CellsundifferentiatedHuman Embryonic Stem CellsadultForeskin Cells ParentalundifferentiatediPS cells from episomal vectors (Defined Factor)undifferentiatediPS cells from episomal vectors (Defined Factor sub-clone)undifferentiatediPS cells from episomal vectors (Defined Factor sub-clone)undifferentiatediPS cells from episomal vectors (Defined Factor sub-clone)undifferentiatediPS cells from episomal vectors (Defined Factor)undifferentiatedadipose stem cells derived from adult human donoradultadipose stem cells derived from adult human donoradultadipose stem cells derived from adult human donorundifferentiatediPSC derived from hASC using lentiviral reprogramming vectorundifferentiatediPSC derived from hASC using lentiviral reprogramming vectorundifferentiatediPSC derived from hASC using lentiviral reprogramming vectorundifferentiatediPSC derived from hASC using minicircle reprogramming vectorundifferentiatediPSC derived from hASC using minicircle reprogramming vectorundifferentiatediPSC derived from hASC using minicircle reprogramming vector355904Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced...E-GEOD-20033/profile/8773/arrayexpressuploader219adipose tissuegenomeDec.12, 2014Falseminicircle-derived-ips-cells-affymetrix-dataE-GEOD-20033_A-AFFY-44Minicircle-derived iPS cells - Affymetrix DataSep.15, 2014Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem (iPS) cells. We report the use of ‘minicircle’ DNA, a vector type that is free of bacterial DNA and capable of high expression in cells. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells. (Note: Our Nature Methods publication included analysis of array data from GSM378832 (Foreskin), GSM378833-GSM378838 (JT-iPSC), and GSM378817-GSM378820 (H1, H7, H9, H13, H14) in conjunction with this series). Total RNA from human adipose stem cells (hASC, n = 3 replicate samples), hASC-derived iPS cells using lentiviral factors (lenti-iPSC, n = 3 replicate samples), and minicircle-derived human iPS cells (mc-iPSC, n = 3 subclones from adipose tissue of three individual patients) was hybridized to nine Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20033http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20033/samples/