{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM500988": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "Human Embryonic Stem Cells"}}, {"GSM500988": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "Human Embryonic Stem Cells"}}, {"GSM500988": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "Human Embryonic Stem Cells"}}, {"GSM500988": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "Human Embryonic Stem Cells"}}, {"GSM500995": {"DEVELOPMENTAL STAGE": "adult", "CELL TYPE": "Foreskin Cells Parental"}}, {"GSM500996": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "iPS cells from episomal vectors (Defined Factor)"}}, {"GSM500997": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "iPS cells from episomal vectors (Defined Factor sub-clone)"}}, {"GSM500997": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "iPS cells from episomal vectors (Defined Factor sub-clone)"}}, {"GSM500997": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "iPS cells from episomal vectors (Defined Factor sub-clone)"}}, {"GSM500996": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "iPS cells from episomal vectors (Defined Factor)"}}, {"GSM50100": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "adipose stem cells derived from adult human donor"}}, {"GSM501002": {"DEVELOPMENTAL STAGE": "adult", "CELL TYPE": "adipose stem cells derived from adult human donor"}}, {"GSM501002": {"DEVELOPMENTAL STAGE": "adult", "CELL TYPE": "adipose stem cells derived from adult human donor"}}, {"GSM501004": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "iPSC derived from hASC using lentiviral reprogramming vector"}}, {"GSM501004": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "iPSC derived from hASC using lentiviral reprogramming vector"}}, {"GSM501004": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "iPSC derived from hASC using lentiviral reprogramming vector"}}, {"GSM501007": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "iPSC derived from hASC using minicircle reprogramming vector"}}, {"GSM501007": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "iPSC derived from hASC using minicircle reprogramming vector"}}, {"GSM501007": {"DEVELOPMENTAL STAGE": "undifferentiated", "CELL TYPE": "iPSC derived from hASC using minicircle reprogramming vector"}}], "id": 3559, "pop_total": 0, "platform": 4, "summary_wrapped": "Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced...", "geo_gse_id": "E-GEOD-20033", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 19, "tags": ["adipose tissue", "genome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "minicircle-derived-ips-cells-affymetrix-data", "geo_id_plat": "E-GEOD-20033_A-AFFY-44", "name": "Minicircle-derived iPS cells - Affymetrix Data", "created": "Sep.15, 2014", "summary": "Owing to the risk of insertional mutagenesis, viral transduction has been increasingly replaced by nonviral methods to generate induced pluripotent stem (iPS) cells. We report the use of \u2018minicircle\u2019 DNA, a vector type that is free of bacterial DNA and capable of high expression in cells. Here we use a single minicircle vector to generate transgene-free iPSCs from adult human adipose stem cells. (Note: Our Nature Methods publication included analysis of array data from GSM378832 (Foreskin),  GSM378833-GSM378838 (JT-iPSC), and GSM378817-GSM378820 (H1, H7, H9, H13, H14) in conjunction with this series). Total RNA from human adipose stem cells (hASC, n = 3 replicate samples),  hASC-derived iPS cells using lentiviral factors (lenti-iPSC, n = 3 replicate samples), and minicircle-derived human iPS cells (mc-iPSC, n = 3 subclones from adipose tissue of three individual patients) was hybridized to nine Affymetrix GeneChip Human Genome U133 Plus 2.0 Arrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20033", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-20033/samples/"}