4ArrayExpress Uploader0humannormal growthempty vector controlnormal growthempty vector controlnormal growthRB shRNAnormal growthRB shRNAnormal growthp107 shRNAnormal growthp107 shRNAnormal growthp130 shRNAnormal growthp130 shRNAlow serum quiescenceempty vector controllow serum quiescenceempty vector controllow serum quiescenceRB shRNAlow serum quiescenceRB shRNAlow serum quiescencep107 shRNAlow serum quiescencep107 shRNAlow serum quiescencep130 shRNAlow serum quiescencep130 shRNAconfluent quiescenceempty vector controlconfluent quiescenceempty vector controlconfluent quiescenceRB shRNAconfluent quiescenceRB shRNAconfluent quiescencep107 shRNAconfluent quiescencep107 shRNAconfluent quiescencep130 shRNAconfluent quiescencep130 shRNAras-induced senescenceempty vector controlras-induced senescenceempty vector controlras-induced senescenceRB shRNAras-induced senescenceRB shRNAras-induced senescencep107 shRNAras-induced senescencep107 shRNAras-induced senescencep130 shRNAras-induced senescencep130 shRNAnormal growthempty vector controlnormal growthempty vector controlras-induced senescenceempty vector controlras-induced senescenceempty vector controlras-induced senescenceRB shRNAras-induced senescenceRB shRNA3552arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19864The action of RB as a tumor suppressor has been difficult to define, in part, due to the redundancy of the related proteins p107 and...20385362/profile/8773/arrayexpressuploader238cellgenomeserumDec.12, 2014Falsetranscription-profiling-by-array-of-human-imr90-ceE-GEOD-19864_A-AFFY-44Transcription profiling by array of human IMR90 cells with RNAi-mediated suppression of RB, p107 or p130Sep.15, 2014The action of RB as a tumor suppressor has been difficult to define, in part, due to the redundancy of the related proteins p107 and p130. By coupling advanced RNAi technology to suppress RB, p107 or p130 with a genome wide analysis of gene expression in growing, quiescent or ras-senescent cells, we identified a unique and specific activity of RB in repressing DNA replication as cells exit the cell cycle into senescence, a tumor suppressive program. Experiment Overall Design: Expression profiles of IMR90 cells before and after RNAi-mediated supppression of RB, p107 or p130 in growing, quiescent or ras-induced senescent conditions. RNA was extracted from growing, low serum (0.1% FBS), confluent, or ras-senescent cells.E-GEOD-19864http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19864/samples/