{"platform": 4, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM496089": {"growth condition": "normal growth", "genotype": "empty vector control"}}, {"GSM496089": {"growth condition": "normal growth", "genotype": "empty vector control"}}, {"GSM49609": {"growth condition": "normal growth", "genotype": "RB shRNA"}}, {"GSM49609": {"growth condition": "normal growth", "genotype": "RB shRNA"}}, {"GSM496093": {"growth condition": "normal growth", "genotype": "p107 shRNA"}}, {"GSM496093": {"growth condition": "normal growth", "genotype": "p107 shRNA"}}, {"GSM496095": {"growth condition": "normal growth", "genotype": "p130 shRNA"}}, {"GSM496095": {"growth condition": "normal growth", "genotype": "p130 shRNA"}}, {"GSM496097": {"growth condition": "low serum quiescence", "genotype": "empty vector control"}}, {"GSM496097": {"growth condition": "low serum quiescence", "genotype": "empty vector control"}}, {"GSM496099": {"growth condition": "low serum quiescence", "genotype": "RB shRNA"}}, {"GSM496099": {"growth condition": "low serum quiescence", "genotype": "RB shRNA"}}, {"GSM49610": {"growth condition": "low serum quiescence", "genotype": "p107 shRNA"}}, {"GSM49610": {"growth condition": "low serum quiescence", "genotype": "p107 shRNA"}}, {"GSM496103": {"growth condition": "low serum quiescence", "genotype": "p130 shRNA"}}, {"GSM496103": {"growth condition": "low serum quiescence", "genotype": "p130 shRNA"}}, {"GSM496105": {"growth condition": "confluent quiescence", "genotype": "empty vector control"}}, {"GSM496105": {"growth condition": "confluent quiescence", "genotype": "empty vector control"}}, {"GSM496107": {"growth condition": "confluent quiescence", "genotype": "RB shRNA"}}, {"GSM496107": {"growth condition": "confluent quiescence", "genotype": "RB shRNA"}}, {"GSM496109": {"growth condition": "confluent quiescence", "genotype": "p107 shRNA"}}, {"GSM496109": {"growth condition": "confluent quiescence", "genotype": "p107 shRNA"}}, {"GSM496": {"growth condition": "confluent quiescence", "genotype": "p130 shRNA"}}, {"GSM496": {"growth condition": "confluent quiescence", "genotype": "p130 shRNA"}}, {"GSM496113": {"growth condition": "ras-induced senescence", "genotype": "empty vector control"}}, {"GSM496113": {"growth condition": "ras-induced senescence", "genotype": "empty vector control"}}, {"GSM496115": {"growth condition": "ras-induced senescence", "genotype": "RB shRNA"}}, {"GSM496115": {"growth condition": "ras-induced senescence", "genotype": "RB shRNA"}}, {"GSM496117": {"growth condition": "ras-induced senescence", "genotype": "p107 shRNA"}}, {"GSM496117": {"growth condition": "ras-induced senescence", "genotype": "p107 shRNA"}}, {"GSM496119": {"growth condition": "ras-induced senescence", "genotype": "p130 shRNA"}}, {"GSM496119": {"growth condition": "ras-induced senescence", "genotype": "p130 shRNA"}}, {"GSM496089": {"growth condition": "normal growth", "genotype": "empty vector control"}}, {"GSM496089": {"growth condition": "normal growth", "genotype": "empty vector control"}}, {"GSM496113": {"growth condition": "ras-induced senescence", "genotype": "empty vector control"}}, {"GSM496113": {"growth condition": "ras-induced senescence", "genotype": "empty vector control"}}, {"GSM496115": {"growth condition": "ras-induced senescence", "genotype": "RB shRNA"}}, {"GSM496115": {"growth condition": "ras-induced senescence", "genotype": "RB shRNA"}}], "id": 3552, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19864", "summary_wrapped": "The action of RB as a tumor suppressor has been difficult to define, in part, due to the redundancy of the related proteins p107 and...", "pubmed_id": 20385362, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 38, "tags": ["cell", "genome", "serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-human-imr90-ce", "geo_id_plat": "E-GEOD-19864_A-AFFY-44", "name": "Transcription profiling by array of human IMR90 cells with RNAi-mediated suppression of RB, p107 or p130", "created": "Sep.15, 2014", "summary": "The action of RB as a tumor suppressor has been difficult to define, in part, due to the redundancy of the related proteins p107 and p130. By coupling advanced RNAi technology to suppress RB, p107 or p130 with a genome wide analysis of gene expression in growing, quiescent or ras-senescent cells, we identified a unique and specific activity of RB in repressing DNA replication as cells exit the cell cycle into senescence, a tumor suppressive program. Experiment Overall Design: Expression profiles of IMR90 cells before and after RNAi-mediated supppression of RB, p107 or p130 in growing, quiescent or ras-induced senescent conditions. RNA was extracted from growing, low serum (0.1% FBS), confluent, or ras-senescent cells.", "geo_gse_id": "E-GEOD-19864", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19864/samples/"}