{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3545, "factors": [{"GSM494403": {"HSF1": "wild-type"}}, {"GSM494404": {"HSF1": "chicken HSF1"}}, {"GSM494405": {"HSF1": "mutated human HSF1"}}, {"GSM494405": {"HSF1": "mutated human HSF1"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "To analyze target genes of human heat shock transcription factor 1 (HSF1), we first generated two independent HeLa clones (RDT1 and RDT2)...", "pubmed_id": 20834230, "geo_gse_id": "E-GEOD-19797", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["amino acid", "cell", "genome", "glutamic acid", "leucine"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "identification-of-genes-up-regulated-by-the-overex", "geo_id_plat": "E-GEOD-19797_A-AFFY-44", "name": "Identification of genes up-regulated by the overexpression of HSF1 in human HeLa cells", "created": "Sep.15, 2014", "summary": "To analyze target genes of human heat shock transcription factor 1 (HSF1), we first generated two independent HeLa clones (RDT1 and RDT2) expressing an actively mutated hHSF1 (hHSF1\u0394RDT), which lacks the regulatory domain that masks its activation domain and possesses a glutamic acid at amino acid 395 instead of a leucine in the suppression domain of the trimerization domain (Fujimoto et al., J. Biol. Chem. 280, 34908-34916, 2005). We also generated a HeLa clone expressing chicken HSF1 (HeLa/cHSF1) to compare its profile of gene expression with those of RDT1 and RDT2 cells (Nakai and Morimoto, Mol. Cell. Biol. 13, 1983-1997, 1993). We then carried out DNA microarray analysis using total RNA isolated from HeLa, HeLa/cHSF1, RDT1, and RDT2 cells grown under normal growth conditions. mRNA levels in human HeLa, RDT1, RDT2, and HeLa/cHSF1 were analyzed by DNA microarray analysis using GeneChip Human Genome U133 Plus 2.0 Array (Affymetrix).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19797", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19797/samples/"}