{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM493918": {"TREATMENT": "control"}}, {"GSM493918": {"TREATMENT": "control"}}, {"GSM493918": {"TREATMENT": "control"}}, {"GSM49392": {"TREATMENT": "si221"}}, {"GSM49392": {"TREATMENT": "si221"}}, {"GSM49392": {"TREATMENT": "si221"}}, {"GSM493924": {"TREATMENT": "si222"}}, {"GSM493924": {"TREATMENT": "si222"}}, {"GSM493924": {"TREATMENT": "si222"}}], "id": 3544, "pop_total": 0, "platform": 4, "summary_wrapped": "Full title: Expression data from antisense miRNA-221/222 (si221/222) and control inhibitor (GFP) treated fulvestrant-resistant breast...", "geo_gse_id": "E-GEOD-19777", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": ["breast", "breast cancer", "cancer", "cell", "line"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-19777_A-AFFY-44", "slug": "antisense-mirna-221222-si221222-and-control-inhibi", "geo_gds_id": "", "name": "Antisense miRNA-221/222 (si221/222) and control inhibitor (GFP) treated fulvestrant-resistant breast cancer cells", "created": "Sep.15, 2014", "summary": "Full title: Expression data from antisense miRNA-221/222 (si221/222) and control inhibitor (GFP) treated fulvestrant-resistant breast cancer cells The expression of miR-221/222 were found to be upregulated in fulvestrant resistant breast cancer cells MCF7-FR compared to its drug-sensitive counterpart MCF7. To investigate the role of miR-221/222 in acquired resistance to fulvestrant, we lowered the level of miR-221/222 in MCF7-FR cells using miRNA inhibitors (antagomirs), and compared gene expression profiles before and after treatment. Fulvestrant-resistant breast cancer cells MCF7-FR (originated from drug-sentitive breast cancer model cell line MCF7) were transient-transfected by antigomirs targeting miR221 or  miR222 (i.e. si221, si222).  All three cell lines, MCF7-FR, siR221, siRNA222 were subjected to gene expression profiling.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19777", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19777/samples/"}