{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM492826": {"CELL TYPE": "Embryonic Stem Cells (hESCs)", "DEVELOPMENTAL STAGE": "embroynic"}}, {"GSM492826": {"CELL TYPE": "Embryonic Stem Cells (hESCs)", "DEVELOPMENTAL STAGE": "embroynic"}}, {"GSM492828": {"CELL TYPE": "hESC-derived Endothelial Cells", "DEVELOPMENTAL STAGE": "embroynic"}}, {"GSM492828": {"CELL TYPE": "hESC-derived Endothelial Cells", "DEVELOPMENTAL STAGE": "embroynic"}}, {"GSM492828": {"CELL TYPE": "hESC-derived Endothelial Cells", "DEVELOPMENTAL STAGE": "embroynic"}}, {"GSM492828": {"CELL TYPE": "hESC-derived Endothelial Cells", "DEVELOPMENTAL STAGE": "embroynic"}}, {"GSM492832": {"CELL TYPE": "umbilical vein endothelial cell", "DEVELOPMENTAL STAGE": "mature"}}, {"GSM492832": {"CELL TYPE": "umbilical vein endothelial cell", "DEVELOPMENTAL STAGE": "mature"}}, {"GSM492832": {"CELL TYPE": "umbilical vein endothelial cell", "DEVELOPMENTAL STAGE": "mature"}}, {"GSM492832": {"CELL TYPE": "umbilical vein endothelial cell", "DEVELOPMENTAL STAGE": "mature"}}, {"GSM492836": {"CELL TYPE": "Cord Blood CD34+ Cells", "DEVELOPMENTAL STAGE": "mature"}}, {"GSM492836": {"CELL TYPE": "Cord Blood CD34+ Cells", "DEVELOPMENTAL STAGE": "mature"}}], "id": 3542, "pop_total": 0, "platform": 4, "summary_wrapped": "The pathways involved in hierarchical differentiation of human embryonic stem cells (hESC) into abundant and durable endothelial cells...", "pubmed_id": 20081865, "geo_gse_id": "E-GEOD-19735", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 12, "tags": ["cadherin", "cell", "embryonic stem cell", "line", "serum", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-19735_A-AFFY-44", "slug": "comparison-of-human-embroynic-stem-cell-derived-va", "geo_gds_id": "", "name": "Comparison of human embroynic stem cell derived vascular cells to mature human vascular and hematopoietic cells", "created": "Sep.15, 2014", "summary": "The pathways involved in hierarchical differentiation of human embryonic stem cells (hESC) into abundant and durable endothelial cells (EC) are unknown. We employed an EC-specific VE-cadherin promoter driving GFP (hVPr-GFP) to screen for factors that augmented yields of vascular-committed ECs from hESCs. In phase 1 of our approach, inhibition of TGFb, precisely at day 7 of hESC differentiation, enhanced emergence of hVPr-GFP+ ECs by 10-fold. In the second phase, TGFb-inhibition preserved proliferation and vascular identity of purified ECs, resulting in net 36-fold expansion of homogenous EC-monolayers, and allowing transcriptional profiling that revealed a unique angiogenic signature defined by the VEGFR2highId1highVE-cadherin+EphrinB2+CD133+HoxA9- phenotype. Using an Id1-YFP hESC reporter line, we showed that TGFb-inhibition sustained Id1 expression in hESC-derived ECs, which was required for increased proliferation and preservation of EC commitment. These data provide a multiphasic method for serum-free differentiation and long-term maintenance of authentic hESC-derived ECs, establishing clinical-scale generation of transplantable human ECs. The experiment compared 6 sets of biological duplicates which included human embryonic stem cell derived and mature vascular cell types.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19735", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19735/samples/"}