ArrayExpress Uploader03532controlMCF-7controlMCF-7controlMCF-7long term estrogen deprivedMCF-7long term estrogen deprivedMCF-7long term estrogen deprivedMCF-7controlZR75-1controlZR75-1controlZR75-1long term estrogen deprivedZR75-1long term estrogen deprivedZR75-1long term estrogen deprivedZR75-1controlMDA-361controlMDA-361controlMDA-361long term estrogen deprivedMDA-361long term estrogen deprivedMDA-361long term estrogen deprivedMDA-361controlHCC-1428controlHCC-1428controlHCC-1428long term estrogen deprivedHCC-1428long term estrogen deprivedHCC-1428long term estrogen deprivedHCC-1428arrayexpress_sid4Hyperactivation of phosphatidylinositol-3 kinase (PI3K) promotes escape from hormone dependence in estrogen receptor-positive breast...20530877E-GEOD-19639/profile/8773/arrayexpressuploader224breastbreast cancercancercellestrogenhcchormonephosphatidylinositolserumDec.12, 2014FalseE-GEOD-19639_A-AFFY-44transcription-profiling-by-array-of-human-estrogenTranscription profiling by array of human estrogen receptor positive breast cancer cell lines after long-term estrogen deprivationSep.15, 2014Hyperactivation of phosphatidylinositol-3 kinase (PI3K) promotes escape from hormone dependence in estrogen receptor-positive breast cancer. A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. We used gene expression microarrays to identify genes and pathways that are commonly dysregulated in ER+ cell lines with acquired hormone-independent growth. MCF-7, ZR75-1, MDA-361, and HCC-1428 ER+, estrogen-responsive breast cancer cells were cultured under hormone-depleted conditions (10% DCC-FBS) for several months until sustainable hormone-independent cell populations emerged. Parental and long-term estrogen-deprived (LTED) cells were treated with 10% dextran-coated charcoal-treated fetal bovine serum (DCC-FBS) x 24 hrs prior to RNA harvest for array analysis.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19639humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19639/samples/