{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3532, "factors": [{"GSM490043": {"growth condition": "control", "cell line": "MCF-7"}}, {"GSM490043": {"growth condition": "control", "cell line": "MCF-7"}}, {"GSM490043": {"growth condition": "control", "cell line": "MCF-7"}}, {"GSM490046": {"growth condition": "long term estrogen deprived", "cell line": "MCF-7"}}, {"GSM490046": {"growth condition": "long term estrogen deprived", "cell line": "MCF-7"}}, {"GSM490046": {"growth condition": "long term estrogen deprived", "cell line": "MCF-7"}}, {"GSM490049": {"growth condition": "control", "cell line": "ZR75-1"}}, {"GSM490049": {"growth condition": "control", "cell line": "ZR75-1"}}, {"GSM490049": {"growth condition": "control", "cell line": "ZR75-1"}}, {"GSM490052": {"growth condition": "long term estrogen deprived", "cell line": "ZR75-1"}}, {"GSM490052": {"growth condition": "long term estrogen deprived", "cell line": "ZR75-1"}}, {"GSM490052": {"growth condition": "long term estrogen deprived", "cell line": "ZR75-1"}}, {"GSM490055": {"growth condition": "control", "cell line": "MDA-361"}}, {"GSM490055": {"growth condition": "control", "cell line": "MDA-361"}}, {"GSM490055": {"growth condition": "control", "cell line": "MDA-361"}}, {"GSM490058": {"growth condition": "long term estrogen deprived", "cell line": "MDA-361"}}, {"GSM490058": {"growth condition": "long term estrogen deprived", "cell line": "MDA-361"}}, {"GSM490058": {"growth condition": "long term estrogen deprived", "cell line": "MDA-361"}}, {"GSM49006": {"growth condition": "control", "cell line": "HCC-1428"}}, {"GSM49006": {"growth condition": "control", "cell line": "HCC-1428"}}, {"GSM49006": {"growth condition": "control", "cell line": "HCC-1428"}}, {"GSM490064": {"growth condition": "long term estrogen deprived", "cell line": "HCC-1428"}}, {"GSM490064": {"growth condition": "long term estrogen deprived", "cell line": "HCC-1428"}}, {"GSM490064": {"growth condition": "long term estrogen deprived", "cell line": "HCC-1428"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Hyperactivation of phosphatidylinositol-3 kinase (PI3K) promotes escape from hormone dependence in estrogen receptor-positive breast...", "pubmed_id": 20530877, "geo_gse_id": "E-GEOD-19639", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 24, "tags": ["breast", "breast cancer", "cancer", "cell", "estrogen", "hcc", "hormone", "phosphatidylinositol", "serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-19639_A-AFFY-44", "slug": "transcription-profiling-by-array-of-human-estrogen", "geo_gds_id": "", "name": "Transcription profiling by array of human estrogen receptor positive breast cancer cell lines after long-term estrogen deprivation", "created": "Sep.15, 2014", "summary": "Hyperactivation of phosphatidylinositol-3 kinase (PI3K) promotes escape from hormone dependence in estrogen receptor-positive breast cancer.  A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. We used gene expression microarrays to identify genes and pathways that are commonly dysregulated in ER+ cell lines with acquired hormone-independent growth. MCF-7, ZR75-1, MDA-361, and HCC-1428 ER+, estrogen-responsive breast cancer cells were cultured under hormone-depleted conditions (10% DCC-FBS) for several months until sustainable hormone-independent cell populations emerged. Parental and long-term estrogen-deprived (LTED) cells were treated with 10% dextran-coated charcoal-treated fetal bovine serum (DCC-FBS) x 24 hrs prior to RNA harvest for array analysis.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19639", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19639/samples/"}