{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM48197": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "none"}}, {"GSM48197": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "none"}}, {"GSM48197": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "none"}}, {"GSM481974": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "TNP-KLH with incomplete Freund's adjuvant immunization"}}, {"GSM481974": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "TNP-KLH with incomplete Freund's adjuvant immunization"}}, {"GSM481976": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "Peyer's patch", "TREATMENT": "none"}}, {"GSM481976": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "Peyer's patch", "TREATMENT": "none"}}, {"GSM481976": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "Peyer's patch", "TREATMENT": "none"}}, {"GSM48197": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "none"}}, {"GSM48197": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "none"}}, {"GSM48198": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "LPS stimulation"}}, {"GSM48198": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "LPS stimulation"}}, {"GSM481983": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "Pam2CK4 stimulation"}}, {"GSM481983": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "Pam2CK4 stimulation"}}, {"GSM481985": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "RA stimulation"}}, {"GSM48197": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "none"}}, {"GSM481987": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "RA+Pam2CSK4+Pam3CSK4 stimulation"}}, {"GSM481988": {"CELL TYPE": "follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "RA+Pam2CSK4 stimulation"}}, {"GSM562383": {"CELL TYPE": "ex vivo pLN follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "none"}}, {"GSM562384": {"CELL TYPE": "ex vivo pLN follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "CFA immunization"}}, {"GSM562385": {"CELL TYPE": "ex vivo pLN follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "CFA+Pam immunization"}}, {"GSM562386": {"CELL TYPE": "in vitro pLN follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "none"}}, {"GSM562387": {"CELL TYPE": "in vitro pLN  follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "RA+LPS stimulation"}}, {"GSM562388": {"CELL TYPE": "in vitro pLN follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "RA+Pam2CSK4+LPS stimulation"}}, {"GSM562389": {"CELL TYPE": "in vitro pLN follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "Pam3CSK4 stimulation"}}, {"GSM562390": {"CELL TYPE": "in vitro pLN follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "RA+Pam3CSK4 stimulation"}}, {"GSM56239": {"CELL TYPE": "in vitro pLN follicular dendritic cell", "ORGANISM PART": "lymph node", "TREATMENT": "RA+Pam2CSK4+Pam3CSK4 stimulation"}}], "id": 5923, "pop_total": 0, "platform": 6, "summary_wrapped": "Germinal centers (GCs) are clusters of activated B cells built on stromal cells known as follicular dendritic cells (FDCs). In the...", "pubmed_id": 20643338, "geo_gse_id": "E-GEOD-19401", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 27, "tags": ["dendritic", "gut", "immune system", "lymph"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-19401_A-AFFY-45", "slug": "transcription-profiling-by-array-of-mus-musculus-f", "geo_gds_id": "", "name": "Transcription profiling by array of Mus musculus follicular dendritic cells", "created": "Nov.11, 2014", "summary": "Germinal centers (GCs) are clusters of activated B cells built on stromal cells known as follicular dendritic cells (FDCs). In the Peyer\u2019s patches (PPs), GCs are chronically induced by bacteria and are the major sites for generation of gut IgA immune responses. Whether FDCs directly contribute to the IgA production in PP GCs is unknown. To investigate the role FDCs in gut immune system, we examined comprehensive gene profiles of FDCs purified from PPs or perypheral lymph nodes (pLNs) with or without immunization. We also tried to reconstitute the PP FDC signature in vitro by pulsed or continuous stimulation of pLN FDCs through TLRs, RARs or simultaneously through TLRs and RARs. The number of samples is as follows; ex vivo na\u00efve pLN FDC=3, ex vivo immunized pLN FDC=2, ex vivo PP FDCs=3, in vitro without stimulation=2, in vitro with LPS stimulation for 5 hours=1, in vitro with LPS stimulation for 72h=1, in vitro with Pam2CSK4 (Pam) stimulation for 96h=2, in vitro with retinoic acid(RA) stimulation for 96h=2,in vitro with RA+Pam stimulation for 96h=2.  In addition, ex vivo FDCs with or without immunizations were compared (3 samples), or in vitro cultured FDCs with or without various stimulations were compared (6 samples)", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19401", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19401/samples/"}