ArrayExpress Uploader03515G3serous41not specifiedG3serous55not specifiedG3serous61not specifiedG3serous54not specifiedG3serous75not specifiedG3undiff59not specifiedG3serous30not specifiedG3serous38not specifiedG3serous50not specifiedG1serous46not specifiedG3serous42not specifiedG3mixed78not specifiedG2serous34not specifiedG3mullerian42not specifiedG3serous41not specifiedG3serous35not specifiedG3serous67not specifiedG3serous78not specifiedG1serous70not specifiedG3serous56not specifiednot specifiednot specifiednot specifiednot specifiednot specifiednot specifiednot specifiednot specifiednot specifiednot specifiednot specifiedSKOV3not specifiednot specifiednot specifiedOVCAR3not specifiednot specifiednot specifiedOVCA432not specifiednot specifiednot specifiedOAW42not specifiednot specifiednot specifiedIGROV1not specifiednot specifiednot specifiedCABAarrayexpress_sid4Altered phosphatidylcholine (PC) metabolism in epithelial ovarian cancer (EOC) can provide choline-based imaging approaches as powerful...20179205E-GEOD-19352/profile/8773/arrayexpressuploader428cancercellovarian cancerphosphatidylcholineproteinsurfaceDec.12, 2014Falseactivation-of-phosphatidylcholine-cycle-enzymes-inE-GEOD-19352_A-AFFY-44Activation of phosphatidylcholine-cycle enzymes in human epithelial ovarian cancer cellsSep.15, 2014Altered phosphatidylcholine (PC) metabolism in epithelial ovarian cancer (EOC) can provide choline-based imaging approaches as powerful tools to improve diagnosis and identify new therapeutic targets. Biochemical, protein and mRNA expression analyses demonstrated that the increase in the major choline-containing metabolite phosphocholine (PCho) in EOC compared with normal and non-tumoral immortalized counterparts (EONT) mainly rely upon: 1) ChoK activation, consistent with higher protein content and increased ChoK mRNA expression levels; 2) PC-plc activation, consistent with higher, previously reported, protein expression. More limited and variable sources of PCho could derive, in some EOC cells, from activation of Phospholipase D or GPC-pd. Phospholipase A2 activity and isoforms’ expression levels were lower or unchanged in EOC compared with EONT cells. Increased ChoK mRNA, as well as ChoK and PC-plc protein expression, were also detected in surgical specimens isolated from EOC patients. Overall, we demonstrated that the elevated PCho pool detected in EOC cells primarily resulted from the upregulation/activation of ChoK and PC-plc involved in the biosynthetic and in a degradative pathway of the PC-cycle, respectively. 20 EOC frozen surgical specimens, 8 EOC cell lines and Ovarian Surface Epithelial (OSE) cells (4 different preparations, 3 of them pooled in one sample).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19352humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19352/samples/