{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3515, "factors": [{"GSM480502": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "41", "CELL LINE": "not specified"}}, {"GSM480503": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "55", "CELL LINE": "not specified"}}, {"GSM480504": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "61", "CELL LINE": "not specified"}}, {"GSM480505": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "54", "CELL LINE": "not specified"}}, {"GSM480506": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "75", "CELL LINE": "not specified"}}, {"GSM480507": {"GRADE": "G3", "HISTOTYPE": "undiff", "AGE AT DIAGNOSIS": "59", "CELL LINE": "not specified"}}, {"GSM480508": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "30", "CELL LINE": "not specified"}}, {"GSM480509": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "38", "CELL LINE": "not specified"}}, {"GSM480510": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "50", "CELL LINE": "not specified"}}, {"GSM4805": {"GRADE": "G1", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "46", "CELL LINE": "not specified"}}, {"GSM480512": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "42", "CELL LINE": "not specified"}}, {"GSM480513": {"GRADE": "G3", "HISTOTYPE": "mixed", "AGE AT DIAGNOSIS": "78", "CELL LINE": "not specified"}}, {"GSM480514": {"GRADE": "G2", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "34", "CELL LINE": "not specified"}}, {"GSM480515": {"GRADE": "G3", "HISTOTYPE": "mullerian", "AGE AT DIAGNOSIS": "42", "CELL LINE": "not specified"}}, {"GSM480502": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "41", "CELL LINE": "not specified"}}, {"GSM480517": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "35", "CELL LINE": "not specified"}}, {"GSM480518": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "67", "CELL LINE": "not specified"}}, {"GSM480519": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "78", "CELL LINE": "not specified"}}, {"GSM480520": {"GRADE": "G1", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "70", "CELL LINE": "not specified"}}, {"GSM48052": {"GRADE": "G3", "HISTOTYPE": "serous", "AGE AT DIAGNOSIS": "56", "CELL LINE": "not specified"}}, {"GSM480522": {"GRADE": "not specified", "HISTOTYPE": "not specified", "AGE AT DIAGNOSIS": "not specified", "CELL LINE": "not specified"}}, {"GSM480522": {"GRADE": "not specified", "HISTOTYPE": "not specified", "AGE AT DIAGNOSIS": "not specified", "CELL LINE": "not specified"}}, {"GSM480524": {"GRADE": "not specified", "HISTOTYPE": "not specified", "AGE AT DIAGNOSIS": "not specified", "CELL LINE": "SKOV3"}}, {"GSM480525": {"GRADE": "not specified", "HISTOTYPE": "not specified", "AGE AT DIAGNOSIS": "not specified", "CELL LINE": "OVCAR3"}}, {"GSM480526": {"GRADE": "not specified", "HISTOTYPE": "not specified", "AGE AT DIAGNOSIS": "not specified", "CELL LINE": "OVCA432"}}, {"GSM480527": {"GRADE": "not specified", "HISTOTYPE": "not specified", "AGE AT DIAGNOSIS": "not specified", "CELL LINE": "OAW42"}}, {"GSM480528": {"GRADE": "not specified", "HISTOTYPE": "not specified", "AGE AT DIAGNOSIS": "not specified", "CELL LINE": "IGROV1"}}, {"GSM480529": {"GRADE": "not specified", "HISTOTYPE": "not specified", "AGE AT DIAGNOSIS": "not specified", "CELL LINE": "CABA"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Altered phosphatidylcholine (PC) metabolism in epithelial ovarian cancer (EOC) can provide choline-based imaging approaches as powerful...", "pubmed_id": 20179205, "geo_gse_id": "E-GEOD-19352", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 4, "sample_count": 28, "tags": ["cancer", "cell", "ovarian cancer", "phosphatidylcholine", "protein", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "activation-of-phosphatidylcholine-cycle-enzymes-in", "geo_id_plat": "E-GEOD-19352_A-AFFY-44", "name": "Activation of phosphatidylcholine-cycle enzymes in human epithelial ovarian cancer cells", "created": "Sep.15, 2014", "summary": "Altered phosphatidylcholine (PC) metabolism in epithelial ovarian cancer (EOC) can provide choline-based imaging approaches as powerful tools to improve diagnosis and identify new therapeutic targets. Biochemical, protein and mRNA expression analyses demonstrated that the increase in the major choline-containing metabolite phosphocholine (PCho) in EOC compared with normal and non-tumoral immortalized counterparts (EONT) mainly rely upon: 1) ChoK activation, consistent with higher protein content and increased ChoK\uf061 mRNA expression levels; 2) PC-plc activation, consistent with higher, previously reported, protein expression. More limited and variable sources of PCho could derive, in some EOC cells, from activation of Phospholipase D or GPC-pd. Phospholipase A2 activity and isoforms\u2019 expression levels were lower or unchanged in EOC compared with EONT cells. Increased ChoK\uf061 mRNA, as well as ChoK and PC-plc protein expression, were also detected in surgical specimens isolated from EOC patients. Overall, we demonstrated that the elevated PCho pool detected in EOC cells primarily resulted from the upregulation/activation of ChoK and PC-plc involved in the biosynthetic and in a degradative pathway of the PC-cycle, respectively. 20 EOC frozen surgical specimens, 8 EOC cell lines and Ovarian Surface Epithelial (OSE) cells (4 different preparations, 3 of them pooled in one sample).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19352", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19352/samples/"}