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Home › Dataset Library › Expression profiles of villus and crypt layers of large intestine from C57Bl/6, Apc1638N+/-, and p21-/- mice.

Dataset: Expression profiles of villus and crypt layers of large intestine from C57Bl/6, Apc1638N+/-, and p21-/- mice.

Expression profiles obtained from the villus and crypt layers of murine large intestine can elucidate the process of differentiation...

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Expression profiles obtained from the villus and crypt layers of murine large intestine can elucidate the process of differentiation undergone by epithelial cells as they migrate from the undifferentiated bottom of the crypt to the villus tip before being shed into the intestinal lumen. This series includes profiles from wild type mice, as well as mice harboring mutations in genes (APC and p21) which play key roles in the differentiation process. We used microarrays to characterize gene expression profiles at the base of the crypt and at the villus tip of the lumenal layer of the large intestine in order to better understand the process of differentiation and eventual shedding undergone by cells of the large intestinal epithelium. Four wild type, four APC1638+/- and four p21-/- mice were sacrified and the large intestines dissected out. Cells from the villus tip and from the bottom of the crypt were isolated from the lumenal face of the each large intestine using the Weiser method of sequential elution. RNA was extracted from each eluted sample and used to hybridize to Affymetrix 3' expression arrays.

Species:
mouse

Samples:
24

Source:
E-GEOD-19338

PubMed:
20824133

Updated:
Dec.12, 2014

Registered:
Nov.11, 2014


Factors: (via ArrayExpress)
Sample MOUSE GENOTYPE LAYER OF EPITHELIUM
GSM480358 m79 APC- F1 (villus)
GSM480359 n80 APC- F1 (villus)
GSM480360 m05 APC- F1 (villus)
GSM48036 m64 APC- F1 (villus)
GSM480362 m81 C57Bl/6 F1 (villus)
GSM480363 n10 C57Bl/6 F1 (villus)
GSM480364 i28 C57Bl/6 F1 (villus)
GSM480365 h85 C57Bl/6 F1 (villus)
GSM480366 i74 P21- F1 (villus)
GSM480367 j29 P21- F1 (villus)
GSM480368 j04 P21- F1 (villus)
GSM480369 i49 P21- F1 (villus)
GSM480370 m79 APC- F10 (crypt)
GSM48037 n80 APC- F10 (crypt)
GSM480372 m05 APC- F10 (crypt)
GSM480373 m64 APC- F10 (crypt)
GSM480374 m81 C57Bl/6 F10 (crypt)
GSM480375 n10 C57Bl/6 F10 (crypt)
GSM480376 i28 C57Bl/6 F10 (crypt)
GSM480377 h85 C57Bl/6 F10 (crypt)
GSM480378 i74 P21- F10 (crypt)
GSM480379 j29 P21- F10 (crypt)
GSM480380 j04 P21- F10 (crypt)
GSM48038 i49 P21- F10 (crypt)

Tags

  • face
  • intestinal epithelium
  • intestine
  • large intestine
  • villus

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