{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 5909, "factors": [{"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}, {"GSM475957": {"STIMULATION": "not specified", "ANTIBODY": "not specified", "VARIATION": "not specified"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Interleukin-21 (IL-21) is a pleiotropic cytokine that induces expression of transcription factor BLIMP1 (encoded by Prdm1), which...", "pubmed_id": 20064451, "geo_gse_id": "E-GEOD-19198", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 20, "tags": ["cell", "cytokine", "gas", "genome", "interleukin", "plasma cell", "spleen"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "analysis-of-interleukin-21-induced-prdm1-gene-regu", "geo_id_plat": "E-GEOD-19198_A-AFFY-45", "name": "Analysis of interleukin-21-induced Prdm1 gene regulation reveals functional cooperation of STAT3 and IRF4 TFs", "created": "Nov.11, 2014", "summary": "Interleukin-21 (IL-21) is a pleiotropic cytokine that induces expression of transcription factor BLIMP1 (encoded by Prdm1), which regulates plasma cell differentiation and T cell homeostasis. We identified an IL-21 response element downstream of Prdm1 that binds the transcription factors STAT3 and IRF4, which are required for optimal Prdm1 expression. Genome-wide ChIP-Seq mapping of STAT3- and IRF4-binding sites showed that most regions with IL-21-induced STAT3 binding also bound IRF4 in vivo, and furthermore, revealed that the noncanonical TTCnnnTAA GAS motif critical in Prdm1 was broadly used for STAT3 binding. Comparing genome-wide expression array data to binding sites revealed that most IL-21-regulated genes were associated with combined STAT3-IRF4 sites rather than pure STAT3 sites. Correspondingly, ChIP-Seq analysis of Irf4_/_ T cells showed greatly diminished STAT3 binding after IL-21 treatment, and Irf4_/_ mice showed impaired IL- 21-induced Tfh cell differentiation in vivo. These results reveal broad cooperative gene regulation by STAT3 and IRF4. Affymetrix expression data: Prepare CD4+ T cells from spleen. CD4+ T cells were preactivated, rested, and treated with IL-21 for 1, 6, and 24 hours. ChIP-seq data: Profiling of IRF4 and Stat3 binding with and without IL-21 stimulation in wild type and IRF4 KO mice.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19198", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19198/samples/"}