Dataset: Analysis of interleukin-21-induced Prdm1 gene regulation reveals functional cooperation of STAT3 and IRF4 TFs
Interleukin-21 (IL-21) is a pleiotropic cytokine that induces expression of transcription factor BLIMP1 (encoded by Prdm1), which...
Interleukin-21 (IL-21) is a pleiotropic cytokine that induces expression of transcription factor BLIMP1 (encoded by Prdm1), which regulates plasma cell differentiation and T cell homeostasis. We identified an IL-21 response element downstream of Prdm1 that binds the transcription factors STAT3 and IRF4, which are required for optimal Prdm1 expression. Genome-wide ChIP-Seq mapping of STAT3- and IRF4-binding sites showed that most regions with IL-21-induced STAT3 binding also bound IRF4 in vivo, and furthermore, revealed that the noncanonical TTCnnnTAA GAS motif critical in Prdm1 was broadly used for STAT3 binding. Comparing genome-wide expression array data to binding sites revealed that most IL-21-regulated genes were associated with combined STAT3-IRF4 sites rather than pure STAT3 sites. Correspondingly, ChIP-Seq analysis of Irf4_/_ T cells showed greatly diminished STAT3 binding after IL-21 treatment, and Irf4_/_ mice showed impaired IL- 21-induced Tfh cell differentiation in vivo. These results reveal broad cooperative gene regulation by STAT3 and IRF4. Affymetrix expression data: Prepare CD4+ T cells from spleen. CD4+ T cells were preactivated, rested, and treated with IL-21 for 1, 6, and 24 hours. ChIP-seq data: Profiling of IRF4 and Stat3 binding with and without IL-21 stimulation in wild type and IRF4 KO mice.
- Species:
- mouse
- Samples:
- 20
- Source:
- E-GEOD-19198
- PubMed:
- 20064451
- Updated:
- Dec.12, 2014
- Registered:
- Nov.11, 2014
Sample | STIMULATION | ANTIBODY | VARIATION |
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GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |
GSM475957 | not specified | not specified | not specified |