{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 5908, "factors": [{"GSM475930": {"PARENTAL GENOTYPE": "Cbfb+/MYH11 x C57/Bl6", "GENOTYPE": "Cbfb+/MYH11"}}, {"GSM475930": {"PARENTAL GENOTYPE": "Cbfb+/MYH11 x C57/Bl6", "GENOTYPE": "Cbfb+/MYH11"}}, {"GSM475930": {"PARENTAL GENOTYPE": "Cbfb+/MYH11 x C57/Bl6", "GENOTYPE": "Cbfb+/MYH11"}}, {"GSM475930": {"PARENTAL GENOTYPE": "Cbfb+/MYH11 x C57/Bl6", "GENOTYPE": "Cbfb+/MYH11"}}, {"GSM475934": {"PARENTAL GENOTYPE": "Cbfb+/MYH11 x C57/Bl6", "GENOTYPE": "Cbfb+/+"}}, {"GSM475934": {"PARENTAL GENOTYPE": "Cbfb+/MYH11 x C57/Bl6", "GENOTYPE": "Cbfb+/+"}}, {"GSM475934": {"PARENTAL GENOTYPE": "Cbfb+/MYH11 x C57/Bl6", "GENOTYPE": "Cbfb+/+"}}, {"GSM475934": {"PARENTAL GENOTYPE": "Cbfb+/MYH11 x C57/Bl6", "GENOTYPE": "Cbfb+/+"}}, {"GSM475938": {"PARENTAL GENOTYPE": "Cbfb+/- x Cbfb+/-", "GENOTYPE": "Cbfb-/-"}}, {"GSM475938": {"PARENTAL GENOTYPE": "Cbfb+/- x Cbfb+/-", "GENOTYPE": "Cbfb-/-"}}, {"GSM475938": {"PARENTAL GENOTYPE": "Cbfb+/- x Cbfb+/-", "GENOTYPE": "Cbfb-/-"}}, {"GSM47594": {"PARENTAL GENOTYPE": "Cbfb+/- x Cbfb+/-", "GENOTYPE": "Cbfb+/+"}}, {"GSM47594": {"PARENTAL GENOTYPE": "Cbfb+/- x Cbfb+/-", "GENOTYPE": "Cbfb+/+"}}, {"GSM47594": {"PARENTAL GENOTYPE": "Cbfb+/- x Cbfb+/-", "GENOTYPE": "Cbfb+/+"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "It is known that CBFB-MYH11, the fusion gene generated by inversion of chromosome 16 in human acute myeloid leukemia, is causative for...", "pubmed_id": 20007544, "geo_gse_id": "E-GEOD-19194", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 14, "tags": ["acute myeloid leukemia", "chromosome", "leukemia", "myeloid leukemia"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-mus-musculus-e", "geo_id_plat": "E-GEOD-19194_A-AFFY-45", "name": "Transcription profiling by array of Mus musculus expressing Cbfb-MYH11", "created": "Nov.11, 2014", "summary": "It is known that CBFB-MYH11, the fusion gene generated by inversion of chromosome 16 in human acute myeloid leukemia, is causative for oncogenic transformation. However, the mechanism by which CBFB-MYH11 initiates leukemogenesis is not clear. Previously published reports showed that CBFB-MYH11 dominantly inhibits RUNX1 and CBFB, and such inhibition has been suggested as the mechanism for leukemogenesis. However, knockin mice expressing Cbfb-MYH11 (Cbfb+/MYH11) showed defects in primitive hematopoiesis not seen in Cbfb null (Cbfb-/-) embryos indicating that Cbfb-MYH11 has repression independent activities as well. To identify gene expression changes associated with this novel activity, we compared the gene expression profile in the blood cells of Cbfb+/MYH11 and Cbfb-/- embryonic day 12.5 (E12.5) embryos with that of their wildtype littermates. Cbfb-MYH11 chimeras were mated to C57/Bl6 females to generate Cbfb+/MYH11 (Cbfb+/MYH11) and Cbfb+/+ (WT) embryos. Cbfb+/- x Cbfb+/- matings were used to generate Cbfb+/+ (Cbfb+/+) and Cbfb-/- (Cbfb-/-) embryos. Blood from 8-10 E12.5 embryos of the same genotype was pooled, and RNA was isolated, labeled, and hybridized to Affymetrix Genechip mouse microarray (430 2.0) chips. 4 chips were used for both the Cbfb+/MYH11 and littermate control samples. 3 chips were used for the Cbfb-/- samples and littermate control samples.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19194", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19194/samples/"}