{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM474645 1": {}}, {"GSM474646 1": {}}], "id": 8249, "ownerprofile_id": "arrayexpress_sid", "platform": 7, "summary_wrapped": "Understanding the mechanism by which embryonic stem (ES) cells self-renew is critical for the realization of their therapeutic potential....", "geo_gse_id": "E-GEOD-19144", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 2, "tags": ["cell", "serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "id1-maintains-embryonic-stem-cell-self-renewal-by", "geo_id_plat": "E-GEOD-19144_A-AFFY-23", "name": "Id1 maintains embryonic stem cell self-renewal by upregulation of Nanog and repression of Brachyury expression", "created": "Nov.23, 2014", "summary": "Understanding the mechanism by which embryonic stem (ES) cells self-renew is critical for the realization of their therapeutic potential.  Previously it had been shown that in combination with LIF, Id proteins were sufficient to maintain mouse ES cells in a self-renewing state.  Here we investigate the requirement for Id1 in maintaing ES cell self-renewal and blocking differentiation.  We find that Id1-/- ES cells have a propensity to differentiate and a decreased capacity to self-renew.  Chronic or acute loss of Id1 leads to a down-regulation of Nanog, a critical regulator of self-renewal.  In addition, in the absence of Id1, ES cells express elevated levels of Brachyury, a marker of mesendoderm differentiation.  We find that loss of both Nanog and Id1 is required for the up-regulation of Brachyury, and Id1 maintains Nanog expression by blocking the expression of Zeb1, a repressor of Nanog transcription.  These results identify Id1 as an important factor in the maintenance of ES cell self-renewal and suggest a plausible mechanism for its control of lineage commitment. Wild type and Id1-/- ES cells were grown on gelatin under normal self-renewing conditions (in the presence of serum and LIF).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19144", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19144/samples/"}