ArrayExpress Uploader0humanYoung20%Young20%Young20%Old20%Old20%Old20%Young3%Young3%Young3%Old3%Old3%Old3%3494arrayexpress_sid4Senescent is an irreversible form of cell cycle arrest initiated by damaged cell constituents and subsequent pro-oncogenic signaling....E-GEOD-19018/profile/8773/arrayexpressuploader212cellDec.12, 2014Falsechanges-in-gene-expression-of-imr90-cells-with-ageE-GEOD-19018_A-AFFY-44Changes in gene expression of Imr90 cells with age and senescence under 3% and 20% oxygen growth conditionsSep.15, 2014Senescent is an irreversible form of cell cycle arrest initiated by damaged cell constituents and subsequent pro-oncogenic signaling. Replicative senescence in vitro can be considered a model for human aging. When fibroblasts are cultured under atmospheric oxygen conditions of 20%, typical of normal tissue culture procedure, fibroblasts generally reach their replicative capacity at 50-60 population doublings (PDs). When fibroblasts are cultured under normal physiological oxygen conditions of 3%, PDs increase about 30% relative to atmospheric levels. Hence while oxygen is a requirement for normal aerobic respiration, it can contribute to the total amount of oxidative stress to which cells are exposed to, leading to a long-term adverse effect in vitro. Inasmuch, cultures maintained under hyperoxic and hypoxic conditions provide a convenient model system for assessing the relationship between oxygen/oxidative stress and senescence. We used microarrays to profile the changes in global gene expression during aging and senescence of Imr90 cells under growth oxygen conditions of 3% and 20%. Imr90 cells at various population doubling timepoints (young, old, and senescent) grown seperately under 3 and 20% oxygen growth conditions were selected for RNA extraction and hybridization on Affymetrix microarrays. Timepoints from cells grown under 3% and 20% oxygen conditions were age matched via population doublings to ensure accurate cross sample comparison.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19018http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-19018/samples/