{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 3454, "factors": [{"GSE18571GSM462073": {}}, {"GSE18571GSM462074": {}}, {"GSE18571GSM462075": {}}, {"GSE18571GSM462076": {}}, {"GSE18571GSM462077": {}}, {"GSE18571GSM462078": {}}, {"GSE18571GSM462079": {}}, {"GSE18571GSM462080": {}}, {"GSE18571GSM462081": {}}, {"GSE18571GSM462082": {}}, {"GSE18571GSM462083": {}}, {"GSE18571GSM462084": {}}, {"GSE18571GSM462085": {}}, {"GSE18571GSM462086": {}}, {"GSE18571GSM462087": {}}, {"GSE18571GSM462088": {}}, {"GSE18571GSM462089": {}}, {"GSE18571GSM462090": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Mammalian target of rapamycin (mTOR) is a serine/threonine kinase involved in multiple intracellular signaling pathways promoting tumor...", "pubmed_id": 19778445, "geo_gse_id": "E-GEOD-18571", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 18, "tags": ["breast", "breast cancer", "cancer", "cell", "central", "disease", "line", "lymph", "lymph node", "serine", "threonine"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-human-rapamycin-treated", "geo_id_plat": "E-GEOD-18571_A-AFFY-44", "name": "Transcription profiling of human rapamycin treated of MDA-MB-468 breast cancer cell line and MDA-MB-468 xenografts", "created": "Sep.15, 2014", "summary": "Mammalian target of rapamycin (mTOR) is a serine/threonine kinase involved in multiple intracellular signaling pathways promoting tumor growth. mTOR is aberrantly activated in a significant portion of breast cancers and is a promising target for treatment. Rapamycin and its analogues are in clinical trials for breast cancer treatment. Patterns of gene expression (metagenes) may also be used to simulate a biologic process of effects of a drug treatment. In this study, we tested the hypothesis that the gene-expression signature regulated by rapamycin could predict disease outcome for patients with breast cancer. Results: Colony formation and sulforhodamine B (IC50 < 1nM) assays, and xenograft animals showed that MDA-MB-468 cells were sensitive to treatment with rapamycin. The comparison of in vitro and in vivo gene expression data identified a signature, termed rapamycin metagene index (RMI), of 31 genes upregulated by rapamycin treatment in vitro as well as in vivo (false discovery rate of 10%). In the Miller dataset, RMI was significantly associated with tumor size or lymph node status. High (>75) percentile) RMI was significantly associated with longer survival (P = 0.015). On multivariate analysis, RMI (P = 0.029), tumor size (P = 0.015) and lymph node status (P = 0.01) were prognostic. In van 't Veer study, RMI was not associated with the time to develop distant metastasis (P = 0.41). In Wang dataset, RMI predicted time to disease relapse (P = 0.09). Conclusions: Rapamycin-regulated gene expression signature predicts clinical outcome in breast cancer. This supports the central role of mTOR signaling in breast cancer biology and provides further impetus to pursue mTOR-targeted therapies for breast cancer treatment. Mol Cancer. 2009 Sep 24;8(1):75. Experiment Overall Design: Rapamycin treatment of MDA-MB-468 breast cancer cell line: Experiment Overall Design: MDA-MB-468 cell line was treated by DMSO (vehicle) and 100 nM rapamycin for 24 hours. We sought to identify differentially expressed genes. Experiment Overall Design: Rapamycin treatment of breast tumor xenografts: Experiment Overall Design: MDA-MB-468 cells were inoculated in the mammary fat pad of female nude mice. When resulting tumor volumes had reached 75-150 mm3, the mice were divided in four groups. Groups 1 and 2 received a single injection of DMSO (vehicle) or rapamycin (15 mg/kg) intraperitoneally and sacrificied 24 h later (1-day groups). Groups 3 and 4 received weekly injections of DMSO or rapamycin for 3 weeks and sacrificied 24 h after the last injection (22-day groups).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-18571", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-18571/samples/"}