6ArrayExpress Uploaderarrayexpress_sidmouse58320http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-18211Mammalian gonadal sex determination is dependent on proper expression of sex determining genes in fetal gonadal somatic support cells...19864314/profile/8773/arrayexpressuploader012Dec.12, 2014Falsetranscription-profiling-of-mouse-embryonic-day-e-1E-GEOD-18211_A-AFFY-45Transcription profiling of mouse embryonic day (E) 11.5 and E12.5 mouse gonadsNew candidate gene identification for controlling mammalian gonadal sex determinationNov.11, 2014Mammalian gonadal sex determination is dependent on proper expression of sex determining genes in fetal gonadal somatic support cells (i.e., pre-granulosa and pre-Sertoli cells in XX and XY gonads, resp.). We used a unique transgenic mouse strain combined with microarray profiling to identify all the differentially expressed transcripts in XX and XY isolated somatic support cells during critical stages of gonadal development and differentiation. Experiment Overall Design: XX and XY somatic support cells (SSC) were isolated by flow cytometry from embryonic day (E) 11.5 and E12.5 mouse gonads. Total RNA was isolated from pools of isolated cells; 3 pools per sex and each timepoint.E-GEOD-18211http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-18211/samples/