{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM451590": {"genotype": "wild type"}}, {"GSM451590": {"genotype": "wild type"}}, {"GSM451590": {"genotype": "wild type"}}, {"GSM451590": {"genotype": "wild type"}}, {"GSM451594": {"genotype": "Crebbp+/-"}}, {"GSM451594": {"genotype": "Crebbp+/-"}}, {"GSM451594": {"genotype": "Crebbp+/-"}}, {"GSM451594": {"genotype": "Crebbp+/-"}}], "id": 5824, "pop_total": 0, "platform": 6, "summary_wrapped": "Myelodysplastic syndrome (MDS) is considered a disease of hematopoietic stem cell (HSC) origin.  To begin to unravel the molecular...", "pubmed_id": 22198154, "geo_gse_id": "E-GEOD-18061", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 8, "tags": ["bone", "bone marrow", "cell", "disease", "hematopoietic system", "liver", "myelodysplastic syndrome", "stem cell", "syndrome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-hematopoietic", "geo_id_plat": "E-GEOD-18061_A-AFFY-45", "name": "Transcription profiling by array of hematopoietic stem cells from mice heterozygous for CREB binding protein", "created": "Nov.11, 2014", "summary": "Myelodysplastic syndrome (MDS) is considered a disease of hematopoietic stem cell (HSC) origin.  To begin to unravel the molecular mechanisms underlying the deregulation of HSCs in MDS, we performed comparative gene expression profiling on Crebbp+/- and wild type HSCs.  We chose to isolate HSCs from the fetal liver (FLHSC) because at this stage there were no differences in cell number between Crebbp+/- and wild type fetal livers, suggesting no overt hematopoietic differences.  Thus, any change in gene expression found in Crebbp+/- FLHSCs is likely to reflect the initially compromised genetic program of HSC regulation, as opposed to that of Crebbp+/- HSCs in adult bone marrow, where secondary changes in gene expression may also occur as compensatory mechanisms for a compromised or failing hematopoietic system. We used day 14.5 post coitus FLHSC (Sca-1+,Lin-,AA4.1+,c-Kit++) from wild type (wt) and Crebbp heterozygous (ht) embryos to examine changes in gene expression before overt myelodysplastic disease manifestation. Total RNA from wt and Crebbp+/- FLHSCs was isolated, PCR-amplified using the Ovation RNA amplification system and hybridized to Affymetrix Mouse 430 2.0 expression microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-18061", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-18061/samples/"}