{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 8247, "factors": [{"GSM450397 1": {}}, {"GSM450398 1": {}}, {"GSM450399 1": {}}, {"GSM450400 1": {}}, {"GSM450401 1": {}}, {"GSM450402 1": {}}, {"GSM450403 1": {}}], "ownerprofile_id": "arrayexpress_sid", "platform": 7, "summary_wrapped": "Inducible co-stimulator (ICOS) interaction with its ligand (ICOSL) is involved in several T cell effector functions. While blockade of...", "pubmed_id": 19890785, "geo_gse_id": "E-GEOD-17995", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 7, "tags": ["cell", "chemokine", "disease", "spleen", "vein", "volume"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "role-of-icosicosl-interaction-in-acute-gvhd", "geo_id_plat": "E-GEOD-17995_A-AFFY-23", "name": "Role of ICOS:ICOSL interaction in acute GVHD", "created": "Nov.23, 2014", "summary": "Inducible co-stimulator (ICOS) interaction with its ligand (ICOSL) is involved in several T cell effector functions. While blockade of ICOS:ICOSL interaction in chronic graft versus host disease (GVHD) seems benefi cial, results for acute GVHD remain controversial. To further elucidate its role in acute GVHD, C57BL / 6 mice were lethally irradiated and reconstituted with allogeneic spleen cells in the absence or presence of ICOSL-blocking mAb. Mice reconstituted with allogeneic spleen cells experienced severe GVHD and died untreated within 6 \u2013 9 days after transplantation. Mice treated with an anti-ICOSL mAb starting from day 3 after transplantation gained weight again and survived for at least additional 12 days, although the treatment was already stopped at day 11 after transplantation. In contrast, the anti-ICOSL treatment starting from day 0 did not prevent GVHD. The diff erence between therapeutic (day 3) and prophylactic (day 0) anti-ICOSL treatment was independent of CD25 + CD4 + regulatory T cells since their depletion did not abrogate the therapeutic eff ect of ICOSL blockade. Microarray analysis revealed IFN- \u03b3 and chemokine up-regulation in spleen cells of prophylactically treated mice, emphasizing kinetic dependence of acute GVHD modulation via blockade of ICOS:ICOSL interaction. B6 recipients were irradiated with 10.0 Gray, administered from a 137 Cs source. Splenocytes from C3H mice were prepared as single cell suspensions in PBS, depleted of red blood cells and counted. 2 \u2013 3 \u00d7 10^7 C3H splenocytes in a volume of 200 \u03bcl were transplanted into B6 recipients via tail vein injection (4 mice per group per experiment) 4 \u2013 6 h after irradiation. Mice in the treatment group with anti-ICOSL mAb and their respective controls, received 500 \u03bcg mAb i. p starting at day 0 or day 3, followed by subsequent injections of 200 \u03bcg of mAb every other day. At day 4 after transplantation RNA of spleen cells was prepared and subjected to microarray analysis. Combined RNA from allogeneic transplanted mice was hybridized onto 2 independent arrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17995", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17995/samples/"}