ArrayExpress Uploaderarrayexpress_sidhumanWTDMSOWTDMSOWTDMSOWTGDC-0941 (PI3K inhibitor)WTGDC-0941 (PI3K inhibitor)WTGDC-0941 (PI3K inhibitor)PIK3CA.H1047RDMSOPIK3CA.H1047RDMSOPIK3CA.H1047RDMSOPIK3CA.H1047RGDC-0941 (PI3K inhibitor)PIK3CA.H1047RGDC-0941 (PI3K inhibitor)PIK3CA.H1047RGDC-0941 (PI3K inhibitor)339804The PTEN/PI3K pathway is commonly mutated in cancer and therefore represents a rational target for therapeutic intervention. In order to...E-GEOD-17785/profile/8773/arrayexpressuploader212breastcancercellinsulinlineserumDec.12, 2014FalseE-GEOD-17785_A-AFFY-44endogenous-expression-of-an-oncogenic-pi3k-mutatioEndogenous expression of an oncogenic PI3K mutation leads to activated PI3K pathway signaling and an invasive phenotypeSep.15, 2014The PTEN/PI3K pathway is commonly mutated in cancer and therefore represents a rational target for therapeutic intervention. In order to investigate the primary phenotype(s) mediated by mutant PIK3CA in a clean and highly patient-relevant context, we utilized a non-tumorigenic MCF10A parental and isogenic knock-in cell line that harbors a common activating PIK3CA kinase domain mutation (H1047R). We found that introduction of an endogenously mutated PIK3CA primarily results in a marked epithelial-mesenchymal transition (EMT) and invasive phenotype compared to isogenic wild-type cells. Moreover, a potent and selective inhibitor of PIK3CA (GDC-0941) was highly effective and selective on reversing this phenotype compared to cell-proliferation, highlighting a potential new paradigm for studying PI3K-pathway targeted agents. Keywords: Expression Array Gene expression profiles from a parental MCF10A breast line and an MCF10A clone that has a PI3K activating mutation (H1047R) knocked-in. We plated the cells in 10cm dishes overnight in media with serum, but lacking EGF or insulin. DMSO or GDC-0941 (PI3K inhibitor) at an EC50 concentration were added to triplicate plates of cells in the morning. After 4 hours the RNA was harvested using the Qiagen RNeasy kit. There are 12 samples total.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17785http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-17785/samples/