ArrayExpress Uploader0mousecontrolcontrolcontrolcontrolstarvedstarvedstarved8435arrayexpress_sid8C2C12 mouse myoblasts (American Type Culture Collection, Manassas, VA) were maintained in growth medium (DMEM supplemented with 10% Fetal...15608089E-GEOD-1776/profile/8773/arrayexpressuploader17serumDec.12, 2014Falsetranscription-profiling-by-array-of-mouse-myotubesE-GEOD-1776_A-AFFY-36Transcription profiling by array of mouse myotubes grown under control and starvation conditionsNov.24, 2014C2C12 mouse myoblasts (American Type Culture Collection, Manassas, VA) were maintained in growth medium (DMEM supplemented with 10% Fetal Bovine serum, Invitrogen Corporation, Carlsbad, CA) in 5% CO2. Cells were plated at a density of 40-50% in 100mm dishes. When cells reached ~90% confluency 24 hours later, they were switched to differentiation media (DMEM containing 2% horse serum; GIBCO, Invitrogen Corporation, Carlsbad, CA) which was subsequently changed every 48 hours. All cells were allowed to differentiate for 4 days with feeding every two days (day 0, 2, and 4). Beginning at day 4, control cells were re-fed every 48 hours whereas experimental cells were not re-fed. After an additional 4 days (8 days post differentiation), cells were harvested and total RNA was isolated from control and starved (non re-fed cells)http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-1776http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-1776/samples/